ArticlePloS one2020
Effect of crocin and naringenin supplementation in cryopreservation medium on post-thaw rooster sperm quality and expression of apoptosis associated genes.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 58 citations in OpenAlex.
- Mitochondrial Regulation of Spermatozoa Function: Metabolism, Oxidative Stress and Therapeutic Insights.Animals : an open access journal from MDPI · 2025Review
- In vitro assessment of lavender ethanolic extract supplemented semen extender on cryopreserved ram epididymal sperm quality and fertility outcome.BMC veterinary research · 2025Article
- Supplementing Freezing Medium with Crocin Exerts a Protective Effect on Bovine Spermatozoa Through the Modulation of a Heat Shock-Mediated Apoptotic Pathway.Molecules (Basel, Switzerland) · 2025Article
- Letrozole and Crocin: Protecting Leydig Cells and Modulating Androgen Receptor and CYP19 Gene Expression in Busulfan-Induced Azoospermia.Animals : an open access journal from MDPI · 2025Article
- The effect of supplementing rosemary essential oil and selenium yeast in the diet on sperm parameters and antioxidant activity of semen in older broiler breeder roosters.Iranian journal of veterinary research · 2025Article
- From waste to health: exploring the male sexuality enhancing activities of the bioactive components of orange peel.Frontiers in endocrinology · 2025Review
- Effect of crocin and quercetin supplementation in cryopreservation medium on post-thaw human sperm quality.Cell and tissue banking · 2024Article
- Effects of dietary basil (Veterinary and animal science · 2024Article
- Apigenin supplementation substantially improves rooster sperm freezability and post-thaw function.Scientific reports · 2024Article
- Evaluation of the effect of mitoquinone on functional parameters, DNA structure, and genes expression related to the apoptotic and antioxidants of human sperm after freezing-thawing.Molecular biology reports · 2024Article
- Manila duck (Animal reproduction · 2024Article
- New strategies for germ cell cryopreservation: Cryoinjury modulation.Clinical and experimental reproductive medicine · 2023Article
- Effect of Honey, Coenzyme Q10, and β-Carotene/α-Tocopherol as Novel Additives in Rabbit-Sperm Cryopreservation Extender.Animals : an open access journal from MDPI · 2023Article
- Enhancing post-thaw quality of ram epididymal sperm by supplementation of rutin in cryopreservation extender.Scientific reports · 2023Article
- Effect of crocin and treadmill exercise on oxidative stress and heart damage in diabetic rats.PloS one · 2023Article
- Effect of different sources of dietary zinc on sperm quality and oxidative parameters.Frontiers in veterinary science · 2023Article
- Protective effect of rosiglitazone on microscopic and oxidative stress parameters of ram sperm after freeze-thawing.Scientific reports · 2022Article
- Effect of tempol and straw size on rooster sperm quality and fertility after post-thawing.Scientific reports · 2022Article
- Antioxidant Activity and Oxidative Stress-Oriented Apoptosis Pathway in Saccharides Supplemented Cryopreserved Sperm of Pacific Abalone,Antioxidants (Basel, Switzerland) · 2022Article
- Does ergothioneine and thawing temperatures improve rooster semen post-thawed quality?Poultry science · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of our study was to examine the effects of crocin (0.5 (C0.5), 1 (C1) and 1.5 (C1.5) mM) and naringenin (50 (N50), 100 (N100) and 150 (N150) μM) in cryopreservation extender for freezing rooster semen. Sperm motility, viability, abnormalities, membrane functionality, active mitochondria, apoptosis status, lipid peroxidation (LP), GPX, SOD, TAC, the mRNA expression of pro-apoptotic (CASPASE 3) and anti-apoptotic (Bcl-2) genes, fertile eggs, hatched eggs and hatching rate were investigated following freeze-thawing. C1 and N100 resulted in higher (P < 0.05) total motility and progressive motility in comparison to the control group. The C1 and N100 groups improved viability, membrane functionality and reduced lipid peroxidation. We found higher values for active mitochondria with C1 and N100 compared to control group. The C1 and N100 groups showed lower percentages of early apoptosis when compared with control group. Also, C1 and N100 had higher TAC, compared to the control group. The mRNA expressions of BCL-2 in the C1 and N100 groups were significantly higher than that of other treatments. The expression of CASPASES 3 was significantly reduced in C1 and N100 group (P < 0.05) when compared to control group. Significantly higher percentages of fertile eggs, hatched eggs and hatching rate were observed in C1 and N100 compared to the control group. In conclusion, crocin at 1 mM and naringenin at 100 μM seem to improve the post-thawing rooster semen quality, fertility and could protect the sperm by reducing the pro-apoptotic (CASPASE 3) and increasing anti-apoptotic (Bcl-2) genes.
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Registered trials
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