ArticleNano letters2020
Genetically Encoded Phase Contrast Agents for Digital Holographic Microscopy.
Article in Nano letters, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Elucidating the assembly of gas vesicles by systematic protein-protein interaction analysis.The EMBO journal · 2024Article
- Directed Evolution of Acoustic Reporter Genes Using High-Throughput Acoustic Screening.ACS synthetic biology · 2024Article
- Bioorthogonal Labeling Enables In Situ Fluorescence Imaging of Expressed Gas Vesicle Nanostructures.Bioconjugate chemistry · 2024Article
- Recent Advances and Current Trends in Transmission Tomographic Diffraction Microscopy.Sensors (Basel, Switzerland) · 2024Review
- Gas Vesicle-Blood Interactions Enhance Ultrasound Imaging Contrast.Nano letters · 2023Article
- Genomically mined acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing bacteria.Nature biotechnology · 2023Article
- In-vivo programmable acoustic manipulation of genetically engineered bacteria.Nature communications · 2023Article
- Structure of Anabaena flos-aquae gas vesicles revealed by cryo-ET.Structure (London, England : 1993) · 2023Article
- Article
- Dehydration of plant cells shoves nuclei rotation allowing for 3D phase-contrast tomography.Light, science & applications · 2021Article
- Microscopic Object Classification through Passive Motion Observations with Holographic Microscopy.Life (Basel, Switzerland) · 2021Article
- Three-Dimensional Quantitative Intracellular Visualization of Graphene Oxide Nanoparticles by Tomographic Flow Cytometry.Nano letters · 2021Article
- Self-assembly of protein superstructures by physical interactions under cytoplasm-like conditions.Biophysical journal · 2021Article
- Measuring gas vesicle dimensions by electron microscopy.Protein science : a publication of the Protein Society · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Quantitative phase imaging and digital holographic microscopy have shown great promise for visualizing the motion, structure, and physiology of microorganisms and mammalian cells in three dimensions. However, these imaging techniques currently lack molecular contrast agents analogous to the fluorescent dyes and proteins that have revolutionized fluorescence microscopy. Here we introduce the first genetically encodable phase contrast agents based on gas vesicles. The relatively low index of refraction of the air-filled core of gas vesicles results in optical phase advancement relative to aqueous media, making them a "positive" phase contrast agent easily distinguished from organelles, dyes, or microminerals. We demonstrate this capability by identifying and tracking the motion of gas vesicles and gas vesicle-expressing bacteria using digital holographic microscopy, and by imaging the uptake of engineered gas vesicles by mammalian cells. These results give phase imaging a biomolecular contrast agent, expanding the capabilities of this powerful technology for three-dimensional biological imaging.
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Registered trials
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