Evidence map›Paper›PMID 33117859›Full record

ArticleBio-protocol2019

Bacterial Synchronized Transfer Assays in Bone Marrow Derived Macrophages.

Sedelia R Dominguez, Tom Kawula, Shaun P Steele

Abstract read
In one paragraph

Article in Bio-protocol, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sedelia R DominguezPaul G Allen School of Global Animal Health, Washington State University, Pullman, United States.
Tom KawulaPaul G Allen School of Global Animal Health, Washington State University, Pullman, United States.
Shaun P SteelePaul G Allen School of Global Animal Health, Washington State University, Pullman, United States.

Funding

TRAINING IN BIOTECHNOLOGY: EMPHASIS ON PROTEIN CHEMISTRYT32GM008336 · NIGMS · WASHINGTON STATE UNIVERSITY · PI CALL, DOUGLAS R. · 1989 to 2023
$8.9M
Francisella tularensis PathogenesisR01AI082870 · NIAID · WASHINGTON STATE UNIVERSITY · PI KAWULA, THOMAS H · 2010 to 2019
$3.2M
Impact of cytosolic material acquisition by macrophages and dendritic cells on the immune response to intracellular pathogensR56AI139476 · NIAID · WASHINGTON STATE UNIVERSITY · PI KAWULA, THOMAS H · 2019 to 2019
$261k
NIAID NIH HHS R01 AI082870NIAID NIH HHS R56 AI139476NIGMS NIH HHS T32 GM008336
6 · The paper itself

Abstract

Merocytophagy ("mero", Greek for partial; "cytophagy" for cell eating) is a process by which cells acquire microbes and cytosolic material through phagocytosis of a small portion of neighboring cells upon cell-cell contact. Cell-cell contact dependent transfer events can be assessed through co-incubation of differently labeled cells. With these assays, it is difficult to analyze the recipient cells by microscopy or bacterial burden within only recipient cells. Therefore, we established a synchronized transfer assay that allows for recipient cells to be isolated from donor cells following transfer events at a high purity. Here, we present this assay in context of bacterial infections and cytosolic cellular staining. With this protocol, mechanisms of cell-cell contact dependent transfer events and the events following merocytophagy can easily be investigated.

Indexed as

Bacteria transferBone Marrow Derived MacrophagesCell transferMerocytophagyTransfer assay

Identifiers

PMID33117859
PMCPMC7591151

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.