ArticleFrontiers in cell and developmental biology2020
An Immobilization Technique for Long-Term Time-Lapse Imaging of Explanted
Article in Frontiers in cell and developmental biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- A developmental single-cell atlas of the Drosophila visual system glia reveals cell type diversification and subcellular mRNA compartmentalization.PLoS biology · 2026Article
- Cellular quiescence uncouples the proteome from the transcriptome in neural stem cells.The EMBO journal · 2026Article
- Immobilizing Second-InstarCold Spring Harbor protocols · 2025Article
- Lysosomal TPC2 channels disrupt Ca2+ entry and dopaminergic function in models of LRRK2-Parkinson's disease.The Journal of cell biology · 2025Article
- TheeLife · 2025Article
- Licensing and niche competition in spermatogenesis: mathematical models suggest complementary regulation of tissue maintenance.Development (Cambridge, England) · 2025Article
- ThebioRxiv : the preprint server for biology · 2024Article
- Astrocytes control quiescent NSC reactivation via GPCR signaling-mediated F-actin remodeling.Science advances · 2024Article
- Patronin/CAMSAP promotes reactivation and regeneration of Drosophila quiescent neural stem cells.EMBO reports · 2023Article
- Live-Cell Imaging of Drosophila melanogaster Third Instar Larval Brains.Journal of visualized experiments : JoVE · 2023Article
- Ion Channels in Gliomas-From Molecular Basis to Treatment.International journal of molecular sciences · 2023Review
- Live Imaging of the Drosophila Testis Stem Cell Niche.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Photoreceptors generate neuronal diversity in their target field through a Hedgehog morphogen gradient ineLife · 2022Article
- Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants.Journal of visualized experiments : JoVE · 2022Article
- A kinase translocation reporter reveals real-time dynamics of ERK activity in Drosophila.Biology open · 2022Article
- Non-autonomous regulation of neurogenesis by extrinsic cues: aOxford open neuroscience · 2022Review
- A fly's eye view of quiescent neural stem cells.Oxford open neuroscience · 2022Review
- A live-imaging protocol to track cell movement in theSTAR protocols · 2021Article
- Msps governs acentrosomal microtubule assembly and reactivation of quiescent neural stem cells.The EMBO journal · 2021Article
- Histone lysine methyltransferase Pr-set7/SETD8 promotes neural stem cell reactivation.EMBO reports · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Time-lapse imaging is an essential tool to study dynamic biological processes that cannot be discerned from fixed samples alone. However, imaging cell- and tissue-level processes in intact animals poses numerous challenges if the organism is opaque and/or motile. Explant cultures of intact tissues circumvent some of these challenges, but sample drift remains a considerable obstacle. We employed a simple yet effective technique to immobilize tissues in medium-bathed agarose. We applied this technique to study multiple
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.