ArticleJournal of tissue engineering
3D brain tissue physiological model with co-cultured primary neurons and glial cells in hydrogels.
Article in Journal of tissue engineering. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Hydrogels in Neurological Disorders: Emerging Diagnostic and Therapeutic Applications.International journal of nanomedicine · 2026Review
- Application of the Comet Assay in Advanced In Vitro Models.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Hyaluronic acid-based models of the brain microenvironment: Challenges and advances.Current opinion in biomedical engineering · 2025Article
- Development of a Three-Dimensional Pathology-Simulating Model of Neurotrauma Using a Polymer-Encapsulated Neural Cell Network.Gels (Basel, Switzerland) · 2025Article
- Modeling the neuroimmune system in Alzheimer's and Parkinson's diseases.Journal of neuroinflammation · 2024Review
- 3D engineered tissue models for studying human-specific infectious viral diseases.Bioactive materials · 2023Review
- Current progress of cerebral organoids for modeling Alzheimer's disease origins and mechanisms.Bioengineering & translational medicine · 2023Review
- Simulating traumatic brain injuryNeural regeneration research · 2023Review
- Getting closer to modeling the gut-brain axis using induced pluripotent stem cells.Frontiers in cell and developmental biology · 2023Review
- Hydrogel-based 3D human iPSC-derived neuronal culture for the study of rabies virus infection.Frontiers in cellular and infection microbiology · 2023Article
- Tissue-Engineered Models of the Human Brain: State-of-the-Art Analysis and Challenges.Journal of functional biomaterials · 2022Review
- Modelling Human Physiology on-Chip: Historical Perspectives and Future Directions.Micromachines · 2021Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, researchers have focused on the role of gut microbiota on human health and reported the existence of a bidirectional relationship between intestinal microbiota and the brain, referred to as microbiota-gut-brain axis (MGBA). In this context, the development of an organ-on-a-chip platform recapitulating the main players of the MGBA would help in the investigations of the biochemical mechanisms involved. In this work, we focused on the development of a new, hydrogel-based, 3D brain-like tissue model to be hosted in the brain compartment of the aforementioned platform. We previously cultured primary mouse microglial cells, cortical neurons and astrocytes independently, once embedded or covered by a millimeter layer of two selected collagen-based hydrogels. We evaluated cell metabolic activity up to 21 days, cell morphology, spatial distribution and synapse formation. Then, we exploited the best performing culturing condition and developed a more complex brain-like tissue model based on the co-culture of cortical neurons and glial cells in physiological conditions. The obtained results indicate that our 3D hydrogel-based brain tissue model is suitable to recapitulate in vitro the key biochemical parameters of brain tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.