ArticleFrontiers in immunology2020
A Method for Isolating and Culturing Skin Cells: Application to Endothelial Cells, Fibroblasts, Keratinocytes, and Melanocytes From Punch Biopsies in Systemic Sclerosis Skin.
Article in Frontiers in immunology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02562079 (Vasculopathy, Inflammation and Systemic Sclerosis), which is not on this map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Vasculopathy, Inflammation and Systemic Sclerosis: The Role of Endothelial Cell Activation and OX40/OX40L in Modulation of T Lymphocyte Activation
Who cites it
32 citing papers in PubMed, 50 citations in OpenAlex.
- Targeting an IL-17/IL-36 pathogenic hub in hidradenitis suppurativa with a bispecific antibody.Signal transduction and targeted therapy · 2026Trial
- Epithelial interferon-γ responsiveness shapes chemokine induction and clinical outcomes in herpes simplex virus infection.PLoS pathogens · 2026Article
- Antrodia cinnamomea extract exhibits anti-melanogenic and anti-photoaging effects: potential for cosmetic and dermatological applications.Scientific reports · 2026Article
- High-yield isolation and characterization of microvascular niche cells from human full-thickness skin biopsies.Scientific reports · 2026Article
- Review
- An ultrasound-guided biopsy technique for obtaining supraclavicular brown fat biopsies and preadipocytes.Scientific reports · 2025Article
- Bioengineered Skin Grafts from Patient-Derived Decellularized Extracellular Matrix and Autologous Cells for Personalized Regenerative.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Limitations of acyclovir and identification of potent HSV antivirals using 3D bioprinted human skin equivalents.Nature communications · 2025Article
- Standardized Culture of Skin Fibroblasts From Punch Biopsies for Germline DNA Isolation in Myeloid Malignancies: A Practical Bedside-to-Laboratory Approach.Bio-protocol · 2025Article
- Epidermal Keratinocyte Cells in Laboratory, Clinical Trials, and Market: A Narrative Review.Health science reports · 2025Review
- Temperature-Dependent Dynamic Friction of a Single Human Corneocyte at Nanoscale Contact: An Analytical Approach and Direct Measurement Using Lateral Force Microscopy for Haptic Design Insight.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Effects of peptides derived from active sites of visfatin on wound healing.Scientific reports · 2025Article
- The phosphatase PPM1F, a negative regulator of integrin activity, is essential for embryonic development and controls tumor cell invasion.BMC biology · 2025Article
- A lipidomic approach towards identifying the effects of fragrance hydroperoxides on keratinocytes.Contact dermatitis · 2025Article
- Targeting the Epigenome Reduces Keloid Fibroblast Cell Proliferation, Migration, and Invasion.The Journal of investigative dermatology · 2025Article
- Isolation and Culture of Epidermal Melanocytes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Enhancing hair regrowth using rapamycin-primed mesenchymal stem cell-derived exosomes.Theranostics · 2025Article
- Establishing Primary and Stable Cell Lines from Frozen Wing Biopsies for Cellular, Physiological, and Genetic Studies in Bats.Current protocols · 2024Article
- Positive and negative feedback regulation of the TGF-β1 explains two equilibrium states in skin aging.iScience · 2024Article
- Establishing Primary and Stable Cell Lines from Frozen Wing Biopsies for Cellular, Physiological, and Genetic Studies in Bats.bioRxiv : the preprint server for biology · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Systemic Sclerosis (SSc) is a complex auto-immune connective tissue disease combining inflammatory, vasculopathic and fibrotic manifestations. Skin effectively recapitulates the main pathogenic processes and therefore is a good organ to decipher the disease pathophysiology, which remains unclear. However, culturing primary skin cells is SSc can be a major issue due to small sample size combined to skin fibrosis. Here, we present a protocol allowing to isolate and culture the four main types of skin cells: dermal cells (microvascular dermal endothelial cells-HDMECs-and fibroblasts) and epidermal cells (keratinocytes and melanocytes), from a single 4 mm-punch biopsy, at a low cost. The present protocol has been optimized to fit SSc skin cells particularities. Such technique allows to culture primary cells, crucial to study the disease pathophysiology, as well as to isolate cells in order to perform immediate molecular biology experiments such as single-cell transcriptomic. Cells grown from biopsies are also suitable for various types of experiments such as immunocytochemistry, Western blot, RT-qPCR or functional
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.