ArticleScientific reports2020
Human milk microbiota in sub-acute lactational mastitis induces inflammation and undergoes changes in composition, diversity and load.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Bibliometric and visual analysis of human microbiome-breast cancer interactions: current insights and future directions.Frontiers in microbiology · 2024Pooled it
- Microbial Biomarkers of Breast Tumor and Mastitis: Deciphering the Delicate Balance between Potentially "Evil" and "Benign" Alliances in Mammary Microbiomes.Breast care (Basel, Switzerland) · 2026Article
- A One Health Decalogue for Breastfeeding: Microbiota-Targeted Strategies for Infant Gastrointestinal and Neurodevelopmental Health.Nutrients · 2026Review
- Diagnosing Breastfeeding Difficulties: Where Do We Stand?Maternal & child nutrition · 2026Review
- Therapeutic Potential of Thiosemicarbazone Derivative in LPS-Induced Mastitis: Dual Modulation of Inflammatory Response and Blood-Milk Barrier Integrity.Journal of immunology research · 2026Article
- Article
- Maternal knowledge, attitudes, and practices regarding breastfeeding and mastitis.BMC pregnancy and childbirth · 2025Article
- Factors Associated with Anthropometry Z-Scores in Exclusively Breastfed Infants Aged 0-6 Months in 10 Cities of China.Nutrients · 2025Article
- The Role of Targeted Microbiota Therapy in the Prevention and Management of Puerperal Mastitis.Diseases (Basel, Switzerland) · 2025Review
- Investigating the Anna Karenina principle of the breast microbiome.BMC microbiology · 2025Article
- Integrating the milk microbiome signatures in mastitis: milk-omics and functional implications.World journal of microbiology & biotechnology · 2025Review
- Whole-genome sequencing of bacteria accountable for lactational mastitis in humans combined with an examination of their antibiotic resistance profiles.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Immunotherapy in mastitis: state of knowledge, research gaps and way forward.The veterinary quarterly · 2024Review
- Gut and respiratory tract microbiota in children younger than 12 months hospitalized for bronchiolitis compared with healthy children: can we predict the severity and medium-term respiratory outcome?Microbiology spectrum · 2024Article
- The Association Between Lactational Infective Mastitis and the Microbiome: Development, Onset, and Treatments.Cureus · 2024Review
- Article
- Immunological composition of human milk before and during subclinical and clinical mastitis.Frontiers in immunology · 2024Article
- Whole-Genome Sequence of the Multidrug-Resistant Staphylococcus warneri Strain G1M1F, Isolated from Mice with Mastitis.Microbiology resource announcements · 2023Article
- Interrogating the role of the milk microbiome in mastitis in the multi-omics era.Frontiers in microbiology · 2023Review
- Metataxonomic and metaproteomic profiling of the oral microbiome in oral lichen planus - a pilot study.Journal of oral microbiology · 2023Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Sub-acute mastitis (SAM) is a prevalent disease among lactating women, being one of the main reasons for early weaning. Although the etiology and diagnosis of acute mastitis (AM) is well established, little is known about the underlying mechanisms causing SAM. We collected human milk samples from healthy and SAM-suffering mothers, during the course of mastitis and after symptoms disappeared. Total (DNA-based) and active (RNA-based) microbiota were analysed by 16S rRNA gene sequencing and qPCR. Furthermore, mammary epithelial cell lines were exposed to milk pellets, and levels of the pro-inflammatory interleukin IL8 were measured. Bacterial load was significantly higher in the mastitis samples and decreased after clinical symptoms disappeared. Bacterial diversity was lower in SAM milk samples, and differences in bacterial composition and activity were also found. Contrary to AM, the same bacterial species were found in samples from healthy and SAM mothers, although at different proportions, indicating a dysbiotic ecological shift. Finally, mammary epithelial cell exposure to SAM milk pellets showed an over-production of IL8. Our work therefore supports that SAM has a bacterial origin, with increased bacterial loads, reduced diversity and altered composition, which partly recovered after treatment, suggesting a polymicrobial and variable etiology.
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Registered trials
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