ArticleScientific reports2020
Magnetic separation of peripheral nerve-resident cells underscores key molecular features of human Schwann cells and fibroblasts: an immunochemical and transcriptomics approach.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Culture and gene expression profiling of Schwann cells from human sural nerve.Communications biology · 2026Article
- Early injury-induced responses in the transected adult human sural nerve.Research square · 2026Article
- Enhanced nerve regeneration after transplantation of NCAM-positive neural crest-like cells derived from human iPSC.Inflammation and regeneration · 2026Article
- Optogenetic and Endogenous Modulation of CaInternational journal of molecular sciences · 2025Review
- Schwann Cell Development and Myelination.Cold Spring Harbor perspectives in biology · 2024Review
- Identification of cellular and noncellular components of mature intact human peripheral nerve.Journal of the peripheral nervous system : JPNS · 2024Article
- Human Schwann Cells in vitro I. Nerve Tissue Processing, Pre-degeneration, Isolation, and Culturing of Primary Cells.Bio-protocol · 2023Article
- Human Schwann Cells in vitro III. Analytical Methods and a Practical Approach for Quality Control.Bio-protocol · 2023Article
- Human Schwann Cells in vitro II. Passaging, Purification, Banking, and Labeling of Established Cultures.Bio-protocol · 2023Article
- Molecular and spatial landmarks of early mouse skin development.Developmental cell · 2023Article
- Article
- Peripheral nerve-derived fibroblasts promote neurite outgrowth in adult dorsal root ganglion neurons more effectively than skin-derived fibroblasts.Experimental physiology · 2023Article
- Enrichment of Human Dermal Stem Cells from Primary Cell Cultures through the Elimination of Fibroblasts.Cells · 2023Article
- Rats, Neuregulins and Radical Prostatectomy: A Conceptual Overview.Journal of clinical medicine · 2023Review
- A Multi-Stage Bioprocess for the Expansion of Rodent Skin-Derived Schwann Cells in Computer-Controlled Bioreactors.International journal of molecular sciences · 2023Article
- Biomimetic Strategies for Peripheral Nerve Injury Repair: An Exploration of Microarchitecture and Cellularization.Biomedical materials & devices (New York, N.Y.) · 2023Article
- Blood Vessels: The Pathway Used by Schwann Cells to Colonize Nerve Conduits.International journal of molecular sciences · 2022Article
- Transcriptional abnormalities in induced pluripotent stem cell-derived oligodendrocytes of individuals with primary progressive multiple sclerosis.Frontiers in cellular neuroscience · 2022Article
- Activin A Secreted From Peripheral Nerve Fibroblasts Promotes Proliferation and Migration of Schwann Cells.Frontiers in molecular neuroscience · 2022Article
- Nuclear Magnetic Resonance Treatment Accelerates the Regeneration of Dorsal Root Ganglion NeuronsFrontiers in cellular neuroscience · 2022Article
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Authors and funding
11 authors at 3 institutions in 3 countries.
Funding
Abstract
Nerve-derived human Schwann cell (SC) cultures are irreplaceable models for basic and translational research but their use can be limited due to the risk of fibroblast overgrowth. Fibroblasts are an ill-defined population consisting of highly proliferative cells that, contrary to human SCs, do not undergo senescence in culture. We initiated this study by performing an exhaustive immunological and functional characterization of adult nerve-derived human SCs and fibroblasts to reveal their properties and optimize a protocol of magnetic-activated cell sorting (MACS) to separate them effectively both as viable and biologically competent cells. We next used immunofluorescence microscopy imaging, flow cytometry analysis and next generation RNA sequencing (RNA-seq) to unambiguously characterize the post-MACS cell products. High resolution transcriptome profiling revealed the identity of key lineage-specific transcripts and the clearly distinct neural crest and mesenchymal origin of human SCs and fibroblasts, respectively. Our analysis underscored a progenitor- or stem cell-like molecular phenotype in SCs and fibroblasts and the heterogeneity of the fibroblast populations. In addition, pathway analysis of RNA-seq data highlighted putative bidirectional networks of fibroblast-to-SC signaling that predict a complementary, yet seemingly independent contribution of SCs and fibroblasts to nerve regeneration. In sum, combining MACS with immunochemical and transcriptomics approaches provides an ideal workflow to exhaustively assess the identity, the stage of differentiation and functional features of highly purified cells from human peripheral nerve tissues.
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