ArticleNPJ biofilms and microbiomes2020
Intestinal release of biofilm-like microcolonies encased in calcium-pectinate beads increases probiotic properties of Lacticaseibacillus paracasei.
Article in NPJ biofilms and microbiomes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 72 citations in OpenAlex.
- Encapsulation in Calcium Alginate Beads Regulates Growth, Release and Viability of Probiotic Bacteria Through Protective Microenvironments.Gels (Basel, Switzerland) · 2026Article
- Advanced Interpretation of Field Cycling NMR Relaxometry Dispersion Profiles From Hard and Soft Materials.Magnetic resonance in chemistry : MRC · 2026Review
- Research progress on intestinal probiotics-mediated protection of the intestinal mucosal barrier in ulcerative colitis.Frontiers in immunology · 2026Review
- Membrane vesicles produced by next-generation probiotics from the gut as innovative tools for human health.Gut microbes · 2025Review
- Isolation of derivatives from the food-grade probioticGut microbes · 2025Article
- Article
- Novel Encapsulation Approaches in the Functional Food Industry: With a Focus on Probiotic Cells and Bioactive Compounds.Probiotics and antimicrobial proteins · 2025Review
- 3D imaging-driven assembly of multispecies biofilms with antagonistic activity against undesirable bacteria.ISME communications · 2025Article
- Advances in polysaccharide-based biopolymers for probiotic encapsulation: From single polysaccharides to composite systems.Current research in food science · 2025Review
- Modulating the skin mycobiome-bacteriome and treating seborrheic dermatitis with a probiotic-enriched oily suspension.Scientific reports · 2024Article
- Regulatory mechanisms and applications ofFrontiers in microbiology · 2024Review
- Lactobacillus paracasei R3 Alleviates Tumor Progression in Mice with Colorectal Cancer.Current microbiology · 2023Article
- Improved Loading Capacity and Viability of Probiotics Encapsulated in Alginate Hydrogel Beads by In Situ Cultivation Method.Foods (Basel, Switzerland) · 2023Article
- Biofilms and Benign Colonic Diseases.International journal of molecular sciences · 2022Review
- Lactase bacteria in intestinal mucosa are associated with diarrhea caused by high-fat and high-protein diet.BMC microbiology · 2022Article
- Pili and other surface proteins influence the structure and the nanomechanical properties of Lactococcus lactis biofilms.Scientific reports · 2021Article
- Biofilm-based delivery approaches and specific enrichment strategies of probiotics in the human gut.Gut microbesReview
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we show that calcium pectinate beads (CPB) allow the formation of 20 µm spherical microcolonies of the probiotic bacteria Lacticaseibacillus paracasei (formerly designated as Lactobacillus paracasei) ATCC334 with a high cell density, reaching more than 10 log (CFU/g). The bacteria within these microcolonies are well structured and adhere to a three-dimensional network made of calcium-pectinate through the synthesis of extracellular polymeric substances (EPS) and thus display a biofilm-like phenotype, an attractive property for their use as probiotics. During bacterial development in the CPB, a coalescence phenomenon arises between neighboring microcolonies accompanied by their peripheral spatialization within the bead. Moreover, the cells of L. paracasei ATCC334 encased in these pectinate beads exhibit increased resistance to acidic stress (pH 1.5), osmotic stress (4.5 M NaCl), the freeze-drying process and combined stresses, simulating the harsh conditions encountered in the gastrointestinal (GI) tract. In vivo, the oral administration of CPB-formulated L. paracasei ATCC334 in mice demonstrated that biofilm-like microcolonies are successfully released from the CPB matrix in the colonic environment. In addition, these CPB-formulated probiotic bacteria display the ability to reduce the severity of a DSS-induced colitis mouse model, with a decrease in colonic mucosal injuries, less inflammation, and reduced weight loss compared to DSS control mice. To conclude, this work paves the way for a new form of probiotic administration in the form of biofilm-like microcolonies with enhanced functionalities.
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