ArticleJournal of virology2020
Isolation and Characterization of Porcine Astrovirus 5 from a Classical Swine Fever Virus-Infected Specimen.
Article in Journal of virology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- Porcine astrovirus: a review.Archives of virology · 2026Review
- Experimental Porcine Astrovirus 4-Associated Tracheitis and Bronchitis in Pigs.Transboundary and emerging diseases · 2026Article
- Isolation and identification of a new porcine astrovirus 5 demonstrated that oxidative stress enhances porcine astrovirus replication.BMC veterinary research · 2025Article
- Identification of an immunodominant neutralizing epitope of porcine astrovirus type 5 capsid protein.Virologica Sinica · 2025Article
- Isolation and preliminary pathogenicity of a recent feline astrovirus strain in China.Frontiers in veterinary science · 2025Article
- The potential emerging role of piRNA/PIWI complex in virus infection.Virus genes · 2024Review
- Article
- Isolation and Pathogenicity of a Chinese Porcine Astrovirus Type 5 Strain HNPDS-01 and Its Influence on Cecum Microbiota in Piglets.Transboundary and emerging diseases · 2024Article
- Isolation and Molecular Characterization of Atypical Porcine Pestivirus Emerging in China.Viruses · 2023Article
- Replication of Porcine Astrovirus Type 1-Infected PK-15 CellsMicrobiology spectrum · 2023Article
- Multiplex gel-based PCR assay for the simultaneous detection of 5 genotypes of porcine astroviruses.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2023Article
- Viral Coinfections.Viruses · 2022Review
- The Genomic and Genetic Evolution Analysis of Rabbit Astrovirus.Veterinary sciences · 2022Article
- Diversity of Astrovirus in Goats in Southwest China and Identification of Two Novel Caprine Astroviruses.Microbiology spectrum · 2022Article
- Genomic Divergence Characterization and Quantitative Proteomics Exploration of Type 4 Porcine Astrovirus.Viruses · 2022Article
- Epidemiological investigation and genetic characterization of porcine astrovirus genotypes 2 and 5 in Yunnan province, China.Archives of virology · 2022Article
- Mammalian birnaviruses identified in pigs infected by classical swine fever virus.Virus evolution · 2021Article
- Establishment of a Multiplex RT-PCR Method for the Detection of Five Known Genotypes of Porcine Astroviruses.Frontiers in veterinary science · 2021Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many new astroviruses have been identified in humans and other animals in recent years, but only a few have been successfully isolated for extensive biological study. Here, we report an unusual isolation of a porcine astrovirus 5 (PAstV5) strain from a clinical classical swine fever virus (CSFV)-infected tissue sample. Incubation of porcine PK-15 cells with an extract of the CSFV-positive tissue resulted in unexpected cytopathic effects (CPEs), and high-throughput viromic sequencing identified PAstV5 and porcine circovirus type 2 (PCV2) as well as CSFV in the culture. After clearance of CSFV and PCV2, a pure PAstV5 strain, named PAstV5-AH29-2014, was obtained. Analysis revealed virus of typical astroviral morphology with a genome of 6,448 nucleotides, sharing 84.3 to 88.9% nucleotide identity with previously published PAstV5 strains. A mechanistic study showed that CSFV coinfection was likely an important factor for successful isolation by significantly enhancing PAstV5 replication in PK-15 cells via suppression of a type I interferon response. Altogether, PAstV5-AH29-2014, as the first isolated PAstV5 strain, will provide critical material for the investigation of the biological and pathogenic properties of this virus as well as for future development of relevant biological and diagnostic reagents.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.