ArticleBMC microbiology2020
Experimental determination of evolutionary barriers to horizontal gene transfer.
Article in BMC microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Assessing the Ecological Roles of Resistomes within Microbial Communities in Antibiotic-contaminated Ecosystems.Microbial ecology · 2026Review
- Regulatory features determine the evolutionary fate of laterally acquired genes in plants.Molecular biology and evolution · 2026Article
- Hurdles to horizontal gene transfer: species-specific effects of synonymous variation and plasmid copy number determine antibiotic resistance phenotype.Microbiology (Reading, England) · 2026Article
- Emergence of alternative stable states in microbial communities undergoing horizontal gene transfer.eLife · 2025Article
- Unravelling Prokaryotic Codon Usage: Insights from Phylogeny, Influencing Factors and Pathogenicity.Current genomics · 2025Review
- Horizontal Gene Transfer of a key Translation Factor and its Role in Polyproline Proteome Evolution.Molecular biology and evolution · 2024Article
- Comparative In Vitro Activity of Ceftazidime-Avibactam, Imipenem-Relebactam, and Meropenem-Vaborbactam against Carbapenem-Resistant Clinical Isolates ofAntibiotics (Basel, Switzerland) · 2024Article
- Horizontal gene transfer is predicted to overcome the diversity limit of competing microbial species.Nature communications · 2024Article
- Evolutionary barriers to horizontal gene transfer in macrophage-associatedEvolution letters · 2023Article
- Empirical Evidence That Complexity Limits Horizontal Gene Transfer.Genome biology and evolution · 2023Article
- Incompatibility and Interchangeability in Molecular Evolution.Genome biology and evolution · 2023Review
- The Role of the Environment in Horizontal Gene Transfer.Molecular biology and evolution · 2022Article
- Translational demand is not a major source of plasmid-associated fitness costs.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Gene sharing among plasmids and chromosomes reveals barriers for antibiotic resistance gene transfer.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Pseudomonas aeruginosa Pangenome: Core and Accessory Genes of a Highly Resourceful Opportunistic Pathogen.Advances in experimental medicine and biology · 2022Article
- Essential gene acquisition destabilizes plasmid inheritance.PLoS genetics · 2021Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
backgroundHorizontal gene transfer, the acquisition of genes across species boundaries, is a major source of novel phenotypes that enables microbes to rapidly adapt to new environments. How the transferred gene alters the growth - fitness - of the new host affects the success of the horizontal gene transfer event and how rapidly the gene spreads in the population. Several selective barriers - factors that impact the fitness effect of the transferred gene - have been suggested to impede the likelihood of horizontal transmission, however experimental evidence is scarce. The objective of this study was to determine the fitness effects of orthologous genes transferred from Salmonella enterica serovar Typhimurium to Escherichia coli to identify the selective barriers using highly precise experimental measurements.
resultsWe found that most gene transfers result in strong fitness costs. Previously identified evolutionary barriers - gene function and the number of protein-protein interactions - did not predict the fitness effects of transferred genes. In contrast, dosage sensitivity, gene length, and the intrinsic protein disorder significantly impact the likelihood of a successful horizontal transfer.
conclusionWhile computational approaches have been successful in describing long-term barriers to horizontal gene transfer, our experimental results identified previously underappreciated barriers that determine the fitness effects of newly transferred genes, and hence their short-term eco-evolutionary dynamics.
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