ArticleInternational journal of molecular sciences2020
Proteomic Profiling of Two Distinct Populations of Extracellular Vesicles Isolated from Human Seminal Plasma.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 54 citations in OpenAlex.
- Sperm Ion Transporters and Channels in Human Asthenozoospermia: Genetic Etiology, Lessons from Animal Models, and Clinical Perspectives.International journal of molecular sciences · 2022Pooled it
- Seminal Plasma Microbiome Composition and Its Association with Sperm Morphology in Breeding Boars.Biology · 2026Article
- The maternal immune response to semen: implications for reproductive success in livestock.Reproduction & fertility · 2026Review
- Proteomic profiling of porcine seminal extracellular vesicles reveals potential in vivo fertility biomarkers.Andrology · 2026Article
- Heterogeneity in semen-mediated tolerogenic responses: defining immunological determinants of reproductive compatibility.Human reproduction open · 2026Article
- Role of Seminal Exosomes in Reproduction.Journal of cellular physiology · 2025Review
- A functionally tunable magnetic nanochains platform for N-glycoproteomic analysis of extracellular vesicles from ultratrace biofluids.Nature communications · 2025Article
- Seminal Plasma Extracellular Vesicles: Key Mediators of Intercellular Communication in Mammalian Reproductive Systems.Veterinary sciences · 2025Review
- MicroRNA-34a-5p induces cell cycle arrest and leads to spermatogenesis failure by targeting CDC25A.Cellular and molecular life sciences : CMLS · 2025Article
- Protective role of extracellular vesicles against oxidative DNA damage.Biological research · 2025Article
- Human Transmembrane Serine Protease 2 (TMPRSS2) on Human Seminal Fluid Extracellular Vesicles Is Proteolytically Active.Journal of extracellular vesicles · 2025Article
- Advances in extracellular vesicle (EV) biomarkers for precision diagnosis and therapeutic in colorectal cancer.Frontiers in oncology · 2025Review
- Extracellular vesicles: key mediators inFrontiers in veterinary science · 2025Review
- Profiling of urinary extracellular vesicle protein signatures from patients with cribriform and intraductal prostate carcinoma in a cross-sectional study.Scientific reports · 2024Article
- The efficacy and functional consequences of interactions between human spermatozoa and seminal fluid extracellular vesicles.Reproduction & fertility · 2024Article
- Extracellular vesicles from seminal plasma interact with T cells in vitro and drive their differentiation into regulatory T-cells.Journal of extracellular vesicles · 2024Article
- Immunophenotype profile by flow cytometry reveals different subtypes of extracellular vesicles in porcine seminal plasma.Cell communication and signaling : CCS · 2024Article
- Expression of anoctamin 7 (ANO7) is associated with poor prognosis and mucin 2 (MUC2) in colon adenocarcinoma: a study based on TCGA data.Genomics & informatics · 2023Article
- Altered ureido protein modification profiles in seminal plasma extracellular vesicles of non-normozoospermic men.Frontiers in endocrinology · 2023Article
- Seminal extracellular vesicles subsets modulate gene expression in cumulus cells of porcine in vitro matured oocytes.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Body fluids contain many populations of extracellular vesicles (EV) that differ in size, cellular origin, molecular composition, and biological activities. EV in seminal plasma are in majority originating from prostate epithelial cells, and hence are also referred to as prostasomes. Nevertheless, EV are also contributed by other accessory sex glands, as well as by the testis and epididymis. In a previous study, we isolated EV from seminal plasma of vasectomized men, thereby excluding contributions from the testis and epididymis, and identified two distinct EV populations with diameters of 50 and 100 nm, respectively. In the current study, we comprehensively analyzed the protein composition of these two EV populations using quantitative Liquid Chromatography-Mass Spectrometry (LC-MS/MS). In total 1558 proteins were identified. Of these, ≈45% was found only in the isolated 100 nm EV, 1% only in the isolated 50 nm EV, and 54% in both 100 nm and 50 nm EV. Gene ontology (GO) enrichment analysis suggest that both originate from the prostate, but with distinct biogenesis pathways. Finally, nine proteins, including KLK3, KLK2, MSMB, NEFH, PSCA, PABPC1, TGM4, ALOX15B, and ANO7, with known prostate specific expression and alternate expression levels in prostate cancer tissue were identified. These data have potential for the discovery of EV associated prostate cancer biomarkers in blood.
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