ArticlePlants (Basel, Switzerland)2020
Trichostatin A Affects Developmental Reprogramming of Bread Wheat Microspores towards an Embryogenic Route.
Article in Plants (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- Molecular Insights into Microspore Embryogenesis: Transcriptomic Dynamics in Barley and Wheat.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Establishment and maintenance of embryogenic cell fate during microspore embryogenesis.The Plant journal : for cell and molecular biology · 2025Article
- Transcriptomic profiling reveals histone acetylation-regulated genes involved in somatic embryogenesis in Arabidopsis thaliana.BMC genomics · 2024Article
- New Epigenetic Modifier Inhibitors Enhance Microspore Embryogenesis in Bread Wheat.Plants (Basel, Switzerland) · 2024Article
- The improvement of the in vitro plant regeneration in barley with the epigenetic modifier of histone acetylation, trichostatin A.Journal of applied genetics · 2024Article
- Anther Culture Protocols for Barley and Wheat.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Plants and Small Molecules: An Up-and-Coming Synergy.Plants (Basel, Switzerland) · 2023Review
- The Roads to Haploid Embryogenesis.Plants (Basel, Switzerland) · 2023Review
- Microspore embryogenesis induction by mannitol and TSA results in a complex regulation of epigenetic dynamics and gene expression in bread wheat.Frontiers in plant science · 2022Article
- Microtubule organization changes severely after mannitol and n-butanol treatments inducing microspore embryogenesis in bread wheat.BMC plant biology · 2021Article
- Comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (× Triticosecale Wittm.) after 5-azacytidine treatment.Scientific reports · 2021Article
- Review
- Androgenesis-Based Doubled Haploidy: Past, Present, and Future Perspectives.Frontiers in plant science · 2021Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Microspores can be developmentally reprogrammed by the application of different stress treatments to initiate an embryogenic pathway leading to the production of doubled haploid (DH) plants. Epigenetic modifications are involved in cell reprogramming and totipotency in response to stress. To increase microspore embryogenesis (ME) efficiency in bread wheat, the effect of the histone deacetylase inhibitor trichostatin A (TSA) has been examined in two cultivars of wheat with different microspore embryogenesis response. Diverse strategies were assayed using 0-0.4 µM TSA as a single induction treatment and after or simultaneously with cold or mannitol stresses. The highest efficiency was achieved when 0.4 µM TSA was applied to anthers for 5 days simultaneously with a 0.7 M mannitol treatment, producing a four times greater number of green DH plants than mannitol. Ultrastructural studies by transmission electron microscopy indicated that mannitol with TSA and mannitol treatments induced similar morphological changes in early stages of microspore reprogramming, although TSA increased the number of microspores with 'star-like' morphology and symmetric divisions. The effect of TSA on the transcript level of four ME marker genes indicated that the early signaling pathways in ME, involving the
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Registered trials
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