Determination and Dissection of DNA-Binding Specificity for the

Open access · goldAbstract read

In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it

0cells of the map it votes in

3citing papers in PubMed

0.5field-weighted citation impact, top 28% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

A CsoR family transcriptional regulator, TTHA1953, controls the sulfur oxidation pathway in Thermus thermophilus HB8.The Journal of biological chemistry · 2023
Article
Using Restriction Endonuclease, Protection, Selection, and Amplification to Identify Preferred DNA-Binding Sequences of Microbial Transcription Factors.Microbiology spectrum · 2023
Article
Discovering the DNA-Binding Consensus of theInternational journal of molecular sciences · 2021
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Kristi MoncjaDepartment of Chemistry and Biochemistry, Kennesaw State University, Kennesaw, GA 30144, USA.ORCID 0000-0001-8307-2744

Michael W Van DykeDepartment of Chemistry and Biochemistry, Kennesaw State University, Kennesaw, GA 30144, USA.ORCID 0000-0003-0306-4002

Kennesaw State University · US

Funding

Kennesaw State University Foundation Foundation Fellow in BiochemistryNational Science Foundation 1714778

6 · The paper itself

Abstract

Transcription factors (TFs) have been extensively researched in certain well-studied organisms, but far less so in others. Following the whole-genome sequencing of a new organism, TFs are typically identified through their homology with related proteins in other organisms. However, recent findings demonstrate that structurally similar TFs from distantly related bacteria are not usually evolutionary orthologs. Here we explore TTHB099, a cAMP receptor protein (CRP)-family TF from the extremophile

Indexed as

Bacterial ProteinsBinding SitesCyclic AMP Receptor ProteinDNADNA Restriction EnzymesGene DeletionGene Expression Regulation, BacterialPromoter Regions, GeneticProtein BindingSequence Homology, Amino AcidThermus thermophilusBacterial ProteinsCyclic AMP Receptor ProteinDNADNA Restriction Enzymesbioinformaticsbiolayer interferometry (BLI)electrophoretic mobility shift assay (EMSA)extremophileprotein-DNA bindingtype IIS restriction endonuclease

Identifiers

PMID33114549

DOI10.3390/ijms21217929

PMCPMC7662524

OpenAlexW3096918873

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.