Evidence map›Paper›PMID 33113565›Full record

ArticleCardiovascular engineering and technology2020

Transcellular Model for Neutral and Charged Nanoparticles Across an In Vitro Blood-Brain Barrier.

Lin Zhang, Jie Fan, Guanglei Li, Zhaokai Yin, Bingmei M Fu

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cardiovascular engineering and technology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 43 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Review
  6. Review
  7. Review
  8. Review
  9. Article
  10. Review
  11. Review
  12. Article
  13. FeScientific reports · 2024
    Article
  14. Review
  15. Review
  16. Review
  17. Review
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Lin ZhangDepartment of Biomedical Engineering, The City College of the City University of New York, 160 Convent Ave, New York, NY, 10031, USA.
Jie FanDepartment of Biomedical Engineering, The City College of the City University of New York, 160 Convent Ave, New York, NY, 10031, USA.
Guanglei LiDepartment of Biomedical Engineering, The City College of the City University of New York, 160 Convent Ave, New York, NY, 10031, USA.
Zhaokai YinDepartment of Biomedical Engineering, The City College of the City University of New York, 160 Convent Ave, New York, NY, 10031, USA.
Bingmei M FuDepartment of Biomedical Engineering, The City College of the City University of New York, 160 Convent Ave, New York, NY, 10031, USA. fu@ccny.cuny.edu.ORCID 0000-0001-9343-5895
City College of New York · US

Funding

Integrated Microphysiological System of Cerebral Organoid and Blood Vessel for Disease Modeling and Neuropsychiatric Drug screeningUH3TR002151 · NCATS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI LEONG, KAM W · 2020 to 2022
$3.6M
Tumor Cell Arrest and Adhesion in the MicrocirculationSC1CA153325 · NCI · CITY COLLEGE OF NEW YORK · PI FU, BINGMEI M. · 2010 to 2014
$750k
NCATS NIH HHS UH3 TR002151NCI NIH HHS SC1 CA153325
6 · The paper itself

Abstract

purposeThe therapeutic drug-loaded nanoparticles (NPs, 20-100 nm) have been widely used to treat brain disorders. To improve systemic brain delivery efficacy of these NPs, it is necessary to quantify their transport parameters across the blood-brain barrier (BBB) and understand the underlying transport mechanism.

methodsPermeability of an in vitro BBB, bEnd3 (mouse brain microvascular endothelial cells) monolayer, to three neutral NPs with the representative diameters was measured using an automated fluorometer system. To elucidate the transport mechanism of the neutral NPs across the in vitro BBB, and that of positively charged NPs whose BBB permeability was measured in a previous study, we developed a novel transcellular model, which incorporates the charge of the in vitro BBB, the mechanical property of the cell membrane, the ion concentrations of the surrounding salt solution and the size and charge of the NPs.

resultsOur model indicates that the negative charge of the surface glycocalyx and basement membrane of the BBB plays a pivotal role in the transcelluar transport of NPs with diameter 20-100 nm across the BBB. The electrostatic force between the negative charge at the in vitro BBB and the positive charge at NPs greatly enhances NP permeability. The predictions from our transcellular model fit very well with the measured BBB permeability for both neutral and charged NPs.

conclusionOur model can be used to predict the optimal size and charge of the NPs and the optimal charge of the BBB for an optimal systemic drug delivery strategy to the brain.

Indexed as

Capillary PermeabilityDrug CarriersTranscytosisAnimalsBlood-Brain BarrierCell LineElectricityKineticsMiceModels, BiologicalNanoparticlesParticle SizePolystyrenesSurface PropertiesDrug CarriersPolystyrenesbEnd3 (mouse brain microvascular endothelial cells)Blood–brain barrierChargeNanoparticlePermeabilityTranscellular model

Identifiers

PMID33113565
PMCPMC7592456
OpenAlexW3095831640

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.