Evidence map›Paper›PMID 33113109›Full record

ArticleTissue engineering and regenerative medicine2021

A Novel Method to Differentiate Tonsil-Derived Mesenchymal Stem Cells In Vitro into Estrogen-Secreting Cells.

Hee-Yeon Kim, Younghay Lee, Hee-Soo Yoon, Yu-Hee Kim, Kyong-A Cho, So-Youn Woo, Han Sun Kim, Bo-Young Park, Sung-Chul Jung, Inho Jo and 3 more

Erratum issuedOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Tissue engineering and regenerative medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Hee-Yeon Kim *Department of Biochemistry, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea.
Younghay Lee *Department of Biochemistry, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea.
Hee-Soo YoonDepartment of Biochemistry, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea.
Yu-Hee KimDepartment of Microbiology, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea.
Kyong-A ChoDepartment of Microbiology, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea.
So-Youn WooDepartment of Microbiology, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea.
Han Sun KimDepartment of Otolaryngology, College of Medicine, Ewha Womans University, Seoul, 07985, South Korea.
Bo-Young ParkDepartment of Plastic and Reconstructive Surgery, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea.
Sung-Chul JungDepartment of Biochemistry, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea.
Inho JoDepartment of Molecular Medicine, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea.
Woo-Jae ParkDepartment of Biochemistry, College of Medicine, Gachon University, Incheon, 21999, South Korea.
Joo-Won ParkDepartment of Biochemistry, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea. joowon.park@ewha.ac.kr.ORCID http://orcid.org/0000-0002-9765-0477
Kyung-Ha RyuDepartment of Pediatrics, College of Medicine, Ewha Womans University, Seoul, 07804, South Korea. ykh@ewha.ac.kr.
Ewha Womans University · KRGachon University · KR

Funding

Ministry of Education NRF-2017R1E1A1A01073021Ministry of Health and Welfare HI18C2392
6 · The paper itself

Abstract

backgroundThe advantages of tonsil-derived mesenchymal stem cells (TMSCs) over other mesenchymal stem cells (MSCs) include higher proliferation rates, various differentiation potentials, efficient immune-modulating capacity, and ease of obtainment. Specifically, TMSCs have been shown to differentiate into the endodermal lineage. Estrogen deficiency is a major cause of postmenopausal osteoporosis and is associated with higher incidences of ischemic heart disease and cerebrovascular attacks during the postmenopausal period. Therefore, stem cell-derived, estrogen-secreting cells might be used for estrogen deficiency.

methodsHere, we developed a novel method that utilizes retinoic acid, insulin-like growth factor-1, basic fibroblast growth factor, and dexamethasone to evaluate the differentiating potential of TMSCs into estrogen-secreting cells. The efficacy of the novel differentiating method for generation of estrogen-secreting cells was also evaluated with bone marrow- and adipose tissue-derived MSCs.

resultsIncubating TMSCs in differentiating media induced the gene expression of cytochrome P450 19A1 (CYP19A1), which plays a key role in estrogen biosynthesis, and increased 17β-estradiol secretion upon testosterone addition. Furthermore, CYP11A1, CYP17A1, and 3β-hydroxysteroid dehydrogenase type-1 gene expression levels were significantly increased in TMSCs. In bone marrow-derived and adipose tissue-derived MSCs, this differentiation method also induced the gene expression of CYP19A1, but not CYP17A1, suggesting TMSCs are a superior source for estrogen secretion.

conclusionThese results imply that TMSCs can differentiate into functional estrogen-secreting cells, thus providing a novel, alternative cell therapy for estrogen deficiency.

Indexed as

Cell- and Tissue-Based TherapyEstrogensMesenchymal Stem CellsPalatine TonsilCell DifferentiationEstrogensCytochrome P450 family 19 subfamily A member 1EstrogenMesenchymal stem cellSecretionTonsil

Identifiers

PMID33113109
PMCPMC8012432
OpenAlexW3096932673

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.