ArticlePloS one2020
A new perfusion culture method with a self-organized capillary network.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
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- Development of an Easy-to-Fabricate Microdevice for Three-Dimensional Culture and Its Application to Glomerular Endothelial Cell Culture.Micromachines · 2025Article
- Engineering aortic valves via transdifferentiating fibroblasts into valvular endothelial cells without using viruses or iPS cells.Bioactive materials · 2025Article
- In Vitro Model of Vascular Remodeling Under Microfluidic Perfusion.Micromachines · 2024Article
- Cholesterol synthesis is essential for the growth of liver metastasis-prone colorectal cancer cells.Cancer science · 2024Article
- New Strategy for Promoting Vascularization in Tumor Spheroids in a Microfluidic Assay.Advanced healthcare materials · 2023Article
- Hierarchical Vessel Network-Supported Tumor Model-on-a-Chip Constructed by Induced Spontaneous Anastomosis.ACS applied materials & interfaces · 2023Article
- Large-scale perfused tissues via synthetic 3D soft microfluidics.Nature communications · 2023Article
- Improvement of the cell viability of hepatocytes cultured in three-dimensional collagen gels using pump-free perfusion driven by water level difference.Scientific reports · 2022Article
- Transforming Capillary Alginate Gel (Capgel) into New 3D-Printing Biomaterial Inks.Gels (Basel, Switzerland) · 2022Article
- The Vascular Microenvironment in Glioblastoma: A Comprehensive Review.Biomedicines · 2022Review
- Application of Organoids in Carcinogenesis Modeling and Tumor Vaccination.Frontiers in oncology · 2022Review
- Angiogenesis in Free-Standing Two-Vasculature-Embedded Scaffold Extruded by Two-Core Laminar Flow Device.International journal of bioprinting · 2022Article
- Article
- Dynamic 3D On-Chip BBB Model Design, Development, and Applications in Neurological Diseases.Cells · 2021Review
- Three-DimensionalFrontiers in toxicology · 2021Review
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A lack of perfusion has been one of the most significant obstacles for three-dimensional culture systems of organoids and embryonic tissues. Here, we developed a simple and reliable method to implement a perfusable capillary network in vitro. The method employed the self-organization of endothelial cells to generate a capillary network and a static pressure difference for culture medium circulation, which can be easily introduced to standard biological laboratories and enables long-term cultivation of vascular structures. Using this culture system, we perfused the lumen of the self-organized capillary network and observed a flow-induced vascular remodeling process, cell shape changes, and collective cell migration. We also observed an increase in cell proliferation around the self-organized vasculature induced by flow, indicating functional perfusion of the culture medium. We also reconstructed extravasation of tumor and inflammatory cells, and circulation inside spheroids including endothelial cells and human lung fibroblasts. In conclusion, this system is a promising tool to elucidate the mechanisms of various biological processes related to vascular flow.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.