Evidence map›Paper›PMID 33111105›Full record

ArticleSTAR protocols2020

Protocol for Barcoding T Cells Combined with Timed Stimulations.

Kayla R Kulhanek, Darienne R Myers, Olga Ksionda, Yvonne Vercoulen, Damia Romero-Moya, Jeroen P Roose

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Kayla R KulhanekDepartment of Anatomy, University of California San Francisco (UCSF), 513 Parnassus Avenue, Room HSW-1326, San Francisco, CA 94143-0452, USA.
Darienne R MyersDepartment of Anatomy, University of California San Francisco (UCSF), 513 Parnassus Avenue, Room HSW-1326, San Francisco, CA 94143-0452, USA.
Olga KsiondaDepartment of Anatomy, University of California San Francisco (UCSF), 513 Parnassus Avenue, Room HSW-1326, San Francisco, CA 94143-0452, USA.
Yvonne VercoulenDepartment of Anatomy, University of California San Francisco (UCSF), 513 Parnassus Avenue, Room HSW-1326, San Francisco, CA 94143-0452, USA.
Damia Romero-MoyaDepartment of Anatomy, University of California San Francisco (UCSF), 513 Parnassus Avenue, Room HSW-1326, San Francisco, CA 94143-0452, USA.
Jeroen P RooseDepartment of Anatomy, University of California San Francisco (UCSF), 513 Parnassus Avenue, Room HSW-1326, San Francisco, CA 94143-0452, USA.
University of California, San Francisco · US

Funding

Understand the metabolic fitness of naïve T cellsP01AI091580 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI JEROEN ROOSE · 2011 to 2026
$31.1M
Balanced signaling cues to guide cell transitions in the blood lineage continuumR01HL120724 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CHAKRABORTY, ARUP K., NOLAN, GARRY P · 2015 to 2019
$3.7M
Molecular understanding of leukemic bone marrow cytokine-Ras signals and metabolic dependenceR01CA187318 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI JEROEN ROOSE · 2015 to 2026
$3.6M
Loss of Intrinsic Control in Autoimmune T Helper Cells with Signaling VariantsR01AI104789 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ROOSE, JEROEN · 2014 to 2018
$1.9M
NCI NIH HHS R01 CA187318NHLBI NIH HHS R01 HL120724NIAID NIH HHS P01 AI091580NIAID NIH HHS R01 AI104789
6 · The paper itself

Abstract

Stimulation of naive T lymphocytes via the T cell receptor (TCR) induces distinct phosphorylation patterns that can be used to explore various signaling pathways within the cell. This protocol can be used to characterize different perturbations to the signaling pathways and the variations in time of stimulation. Here, we provide a method of barcoding and consolidating a maximum of 24 different sample conditions using two florescent dyes. This single sample for phospho-staining and flow cytometry saves time and reagents. For complete details on the use and execution of this protocol, please refer to Krutzik and Nolan (2006), Krutzik et al. (2012), Vercoulen et al. (2017), Ksionda et al. (2018), and Myers et al. (2019).

Indexed as

Fluorescent DyesMolecular Probe TechniquesT-LymphocytesAnimalsCells, CulturedFlow CytometryMicePhosphorylationReceptors, Antigen, T-CellSignal TransductionTime FactorsFluorescent DyesReceptors, Antigen, T-Cell

Identifiers

PMID33111105
PMCPMC7580249
OpenAlexW3044604752

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.