ArticleSTAR protocols2020
High-Throughput Mass Cytometry Staining for Immunophenotyping Clinical Samples.
Article in STAR protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05414487 (Exploring the Effects of B Cell Depletion With Ofatumumab on Immune Cells and Meningeal Lymphatic Drainage in Patients With Demyelinating Diseases - a Prospective Observational Study), which is not on this map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Exploring the Effects of B Cell Depletion With Ofatumumab on Immune Cells and Meningeal Lymphatic Drainage in Patients With Demyelinating Diseases - a Prospective Observational Study
Who cites it
15 citing papers in PubMed.
- Molecular and cellular features of CTLA-4 blockade for relapsed myeloid malignancies after transplantation.Blood · 2021Trial
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- Immune profiling of age and adjuvant-specific activation of human blood mononuclear cells in vitro.Communications biology · 2024Article
- Spectral Flow Cytometry Methods and Pipelines for Comprehensive Immunoprofiling of Human Peripheral Blood and Bone Marrow.Cancer research communications · 2024Article
- Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline.STAR protocols · 2022Article
- Mass cytometry immunostaining protocol for multiplexing clinical samples.STAR protocols · 2022Article
- Measuring trogocytosis between ovarian tumor and natural killer cells.STAR protocols · 2022Article
- CyTOF mass cytometry analysis of human memory CD4STAR protocols · 2022Article
- Functional analysis of human circulating immune cells based on high-dimensional mass cytometry.STAR protocols · 2022Article
- Expansion, persistence, and efficacy of donor memory-like NK cells infused for posttransplant relapse.The Journal of clinical investigation · 2022Article
- Mass cytometry staining for human bone marrow clinical samples.STAR protocols · 2022Article
- Cisplatin Uptake in Macrophage Subtypes at the Single-Cell Level by LA-ICP-TOFMS Imaging.Analytical chemistry · 2021Article
- Coevolving JAK2V617F+relapsed AML and donor T cells with PD-1 blockade after stem cell transplantation: an index case.Blood advances · 2021Article
- A Randomized Trial of Combined PD-L1 and CTLA-4 Inhibition with Targeted Low-Dose or Hypofractionated Radiation for Patients with Metastatic Colorectal Cancer.Clinical cancer research : an official journal of the American Association for Cancer Research · 2021Article
- Data processing workflow for large-scale immune monitoring studies by mass cytometry.Computational and structural biotechnology journal · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As mass cytometry (MC) is implemented in clinical settings, the need for robust, validated protocols that reduce batch effects between samples becomes increasingly important. Here, we present a streamlined MC workflow for high-throughput staining that generates reproducible data for up to 80 samples in a single experiment by combining reference sample spike-in and palladium-based mass-tag cell barcoding. Although labor intensive, this workflow decreases experimental variables and thus reduces technical error and mitigates batch effects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.