ArticleSTAR protocols2020
Protocol for Isolation of Viable Adult Rat Cardiomyocytes with High Yield.
Article in STAR protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed.
- FGF21-FGFR1 signaling protects against cardiac hypertrophy by regulating PINK1-mediated mitophagy pathway.Journal of advanced research · 2026Article
- Selective Pharmacological Blockade of GPR39 Markedly Reduces No Reflow and Infarct Volumes in a Rat Model of Acute Myocardial Infarction.bioRxiv : the preprint server for biology · 2026Article
- Standardized Isolation, Culture, and Imaging of Contracting Cardiomyocytes from Neonatal Mouse Hearts.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Expression of foetal gene Pontin is essential in protecting heart against pathological remodelling and cardiomyopathy.Nature communications · 2025Article
- An optimized Langendorff-free method for isolation and characterization of primary adult cardiomyocytes.BMC cardiovascular disorders · 2024Article
- In Vivo Mapping of Myocardial Injury Outside the Infarct Zone: Tissue at an Intermediate Pathological State.Journal of the American Heart Association · 2024Article
- An Optimized Langendorff-free Method for Isolation and Characterization of Primary Adult Cardiomyocytes.Research square · 2024Article
- Circulating T cell specific extracellular vesicle profiles in cardiac allograft acute cellular rejection.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2024Article
- Microscopy-based cellular contractility assay for adult, neonatal, and hiPSC cardiomyocytes.STAR protocols · 2022Article
- Application of plasma polymerized pyrrole nanoparticles to prevent or reduce de-differentiation of adult rat ventricular cardiomyocytes.Journal of materials science. Materials in medicine · 2021Article
- Calibration and measurement of mitochondrial pH in intact adult rat cardiomyocytes.STAR protocols · 2021Article
- Protocol for Measurement of Oxygen Consumption RateSTAR protocols · 2020Article
- Protocol for Imaging of Mitoflashes in Live Cardiomyocytes.STAR protocols · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isolation of high-quantity and high-quality ventricular cardiomyocytes from adult rats is critical to study heart physiology and pathology and for drug toxicity screening. It remains challenging to produce a high yield of viable cardiomyocytes from rats. Here, we present our modified enzymatic digestion protocol that relies on the Langendorff device to generate large numbers of viable cardiomyocytes consistently. The most critical parts of this protocol are the selection of rat age and digestion time to obtain viable cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Liu et al. (2019) and Qin et al. (2020).
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Registered trials
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