ArticleBMC microbiology2020
Diversity of mucoid to non-mucoid switch among carbapenemase-producing Klebsiella pneumoniae.
Article in BMC microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 47 citations in OpenAlex.
- Genomic analyses identify nosocomial transmission of ST23 carbapenem-resistant hypervirulentVirulence · 2026Article
- Benefits and costs of a hypercapsule and the mechanism of its loss in a clinical isolate ofmBio · 2026Article
- Genomic dissection of the clonal background and global dissemination of hypervirulent Klebsiella pneumoniae CG23-KL57 lineage.Nature communications · 2026Article
- High-risk extended-spectrum β-lactamase-producing Klebsiella pneumoniae ST307 in a neonatal sepsis outbreak in Zambia: a secondary genomic analysis.Access microbiology · 2026Article
- Epidemiological and Genomic Characteristics ofTransboundary and emerging diseases · 2026Article
- Emergence and hospital dissemination of hypervirulent carbapenem-resistant Klebsiella pneumoniae lineages in eastern China.BMC microbiology · 2025Article
- Potential in-host evolution ofMicrobiology spectrum · 2025Article
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- Insertion sequences accelerate genomic convergence of multidrug resistance and hypervirulence in Klebsiella pneumoniae via capsular phase variation.Genome medicine · 2025Article
- Efficient Photolysis of Multidrug-Resistant Polymicrobial Biofilms.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Bacteriophage Treatment Induces Phenotype Switching and Alters Antibiotic Resistance of ESBLAntibiotics (Basel, Switzerland) · 2025Article
- Adaptive evolution of carbapenem-resistant hypervirulentProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Co-occurrence of ST412mSystems · 2024Article
- Antagonistic Effects ofMicroorganisms · 2024Article
- Development of a novel integrated isothermal amplification system for detection of bacteria-spiked blood samples.AMB Express · 2023Article
- Phages against Noncapsulated Klebsiella pneumoniae: Broader Host range, Slower Resistance.Microbiology spectrum · 2023Article
- Article
- Genetic determinants of host tropism in Klebsiella phages.Cell reports · 2023Article
- Prevalence of hypervirulent and carbapenem-resistantEmerging microbes & infections · 2022Article
- Adaptation to novel spatially-structured environments is driven by the capsule and alters virulence-associated traits.Nature communications · 2022Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundKlebsiella pneumoniae is a leading cause of intractable hospital-acquired multidrug-resistant infections and carbapenemase-producing K. pneumoniae (CPKp) are particularly feared. Most of the clinical isolates produce capsule as a major virulence factor. Recombination events at the capsule locus are frequent and responsible for capsule diversity within Klebsiella spp. Capsule diversity may also occur within clonal bacterial populations generating differences in colony aspect. However, little is known about this phenomenon of phenotypic variation in CPKp and its consequences.
resultsHere, we explored the genetic causes of in vitro switching from capsulated, mucoid to non-mucoid, non-capsulated phenotype in eight clinical CPKp isolates. We compared capsulated, mucoid colony variants with one of their non-capsulated, non-mucoid isogenic variant. The two colony variants were distinguished by their appearance on solid medium. Whole genome comparison was used to infer mutations causing phenotypic differences. The frequency of phenotypic switch was strain-dependent and increased along with colony development on plate. We observed, for 72 non-capsulated variants that the loss of the mucoid phenotype correlates with capsule deficiency and diverse genetic events, including transposition of insertion sequences or point mutations, affecting genes belonging to the capsule operon. Reduced or loss of capsular production was associated with various in vitro phenotypic changes, affecting susceptibility to carbapenem but not to colistin, in vitro biofilm formation and autoaggregation.
conclusionsThe different impact of the phenotypic variation among the eight isolates in terms of capsule content, biofilm production and carbapenem susceptibility suggested heterogeneous selective advantage for capsular loss according to the strain and the mutation. Based on our results, we believe that attention should be paid in the phenotypic characterization of CPKp clinical isolates, particularly of traits related to virulence and carbapenem resistance.
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