Evidence map›Paper›PMID 33108606›Full record

ArticleGlycoconjugate journal2020

Regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene expression during differentiation of human osteoblastic MG-63 cells.

So-Young An, Miri Lee, Hyun-Kyoung Yoon, Fukushi Abekura, Kyoung-Sook Kim, Dong-Hyun Kim, Hyeon-Jun Kim, Kichoon Lee, Cheorl-Ho Kim, Young-Choon Lee

Abstract read
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In one paragraph

Article in Glycoconjugate journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
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  4. Curcumin-mediated transcriptional regulation of humanFrontiers in molecular biosciences · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

So-Young AnDepartment of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan, 49315, South Korea.
Miri LeeDepartment of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan, 49315, South Korea.
Hyun-Kyoung YoonDepartment of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan, 49315, South Korea.
Fukushi AbekuraMolecular and Cellular Glycobiology Unit, Department of Biological Sciences, SungKyunKwan University, Kyunggi-Do, 16419, South Korea.
Kyoung-Sook KimDepartment of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan, 49315, South Korea.
Dong-Hyun KimDepartment of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan, 49315, South Korea.
Hyeon-Jun KimDepartment of Orthopaedic Surgery, College of Medicine, Dong-A University, Busan, 49201, South Korea.
Kichoon LeeFunctional Genomics Laboratory, Department of Animal Sciences, the Ohio State University, 2029 Fyffe Court, Columbus, OH, 43210, USA.
Cheorl-Ho KimMolecular and Cellular Glycobiology Unit, Department of Biological Sciences, SungKyunKwan University, Kyunggi-Do, 16419, South Korea. chkimbio@skku.edu.ORCID 0000-0002-6323-0714
Young-Choon LeeDepartment of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan, 49315, South Korea. yclee@dau.ac.kr.
Dong-A University · KRSungkyunkwan University · KRThe Ohio State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, we found that gene expression of the human β-galactoside α2,6-sialyltransferase (hST6Gal I) was specifically increased during differentiation of human MG-63 osteoblastic cells by serum starvation (SS). In parallel, a distinct increase in binding to SNA, the α2,6-sialyl-specific lectin, was observed in serum-starved cells, as demonstrated by FACS analysis. 5'-Rapid amplification of cDNA ends analysis demonstrated that the increase of hST6Gal I transcript by SS is mediated by P1 promoter. To elucidate transcriptional regulation of hST6Gal I in SS-induced MG-63 cells, we functionally characterized the P1 promoter region of the hST6Gal I gene. The 5'-deletion analysis of P1 promoter region revealed that the 189 bp upstream region of transcription start site is critical for transcriptional activity of hST6Gal I gene in SS-induced MG-63 cells. This region contains the predicted binding sites for several transcription factors, including AREB6, FOXP1, SIX3, HNF1, YY2, and MOK2. The mutagenesis analysis for these sites and chromatin immunoprecipitation assay demonstrated that the YY2 binding site at -98 to -77 was essential for the SS-induced hST6Gal I gene expression during differentiation of MG-63 cells.

Indexed as

Transcription, GeneticAntigens, CDbeta-D-Galactoside alpha 2-6-SialyltransferaseCell DifferentiationDNA-Binding ProteinsEye ProteinsForkhead Transcription FactorsGene Expression Regulation, DevelopmentalGene Expression Regulation, EnzymologicHomeobox Protein SIX3Homeodomain ProteinsHumansNerve Tissue ProteinsOsteoblastsPromoter Regions, GeneticRepressor ProteinsAntigens, CDbeta-D-Galactoside alpha 2-6-SialyltransferaseDNA-Binding ProteinsEye ProteinsForkhead Transcription FactorsFOXP1 protein, humanHomeobox Protein SIX3Homeodomain ProteinsNerve Tissue ProteinsRepressor ProteinsSialyltransferasesST6GAL1 protein, humanTranscription FactorsYY2 protein, humanZEB1 protein, humanZinc Finger E-box-Binding Homeobox 1ZNF239 protein, humanDifferentiationHuman sialyltransferase (hST6Gal I)MG-63 cellsP1 promoterTranscription factor Yin Yang 2 (YY2)

Identifiers

PMID33108606
OpenAlexW3094744879

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.