ArticleNature biotechnology2021
A near-infrared genetically encoded calcium indicator for in vivo imaging.
Article in Nature biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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Who cites it
75 citing papers in PubMed, 140 citations in OpenAlex.
- Photoacoustic and ultrasound Imaging of placental functions in gestational diabetes: From diagnosis to precision interventions.Photoacoustics · 2026Review
- A sensitive orange fluorescent calcium ion indicator for imaging neural activity.Nature communications · 2026Article
- Multiscale photoacoustic imaging of stroke in preclinical models and future directions toward clinical translation [Invited].Biomedical optics express · 2026Review
- Far-red chemigenetic kinase biosensors enable multiplexed and super-resolved imaging of signaling networks.Nature biotechnology · 2026Article
- Multi-scale imaging and recording of in-vivo neural activity.BMB reports · 2026Review
- Photoacoustic Microscopy for Multiscale Biological System Visualization and Clinical Translation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- Imaging the brain by traversing the skull with light and sound.Nature biomedical engineering · 2025Review
- From Microbial Switches to Metabolic Sensors: Rewiring the Gut-Brain Kynurenine Circuit.Biomedicines · 2025Review
- Recent advancements in molecular photoacoustic tomography.JPhys photonics · 2025Review
- Mechanical Modulation, Physiological Roles, and Imaging Innovations of Intercellular Calcium Waves in Living Systems.Cancers · 2025Review
- Reveal the mechanism of brain function with fluorescence microscopy at single-cell resolution: from neural decoding to encoding.Journal of neuroengineering and rehabilitation · 2025Review
- Long-term in vivo immune tracking nanoplatform based on AgBMC biology · 2025Article
- Far-red fluorescent genetically encoded calcium ion indicators.Nature communications · 2025Article
- Nanophotonic-enhanced photoacoustic imaging for brain tumor detection.Journal of nanobiotechnology · 2025Review
- A genetically encoded fluorescent biosensor for visualization of acetyl-CoA in live cells.Cell chemical biology · 2025Article
- Multimodal fluorescence-optoacousticPhotoacoustics · 2025Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Calcium Indicators with Fluorescence Lifetime-Based Signal Readout: A Structure-Function Study.International journal of molecular sciences · 2024Article
- Controlling the sound of light: photoswitching optoacoustic imaging.Nature methods · 2024Review
15 more citing papers are in PubMed but not listed here.
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Authors and funding
15 authors at 5 institutions in 3 countries.
Funding
Abstract
While calcium imaging has become a mainstay of modern neuroscience, the spectral properties of current fluorescent calcium indicators limit deep-tissue imaging as well as simultaneous use with other probes. Using two monomeric near-infrared (NIR) fluorescent proteins (FPs), we engineered an NIR Förster resonance energy transfer (FRET)-based genetically encoded calcium indicator (iGECI). iGECI exhibits high levels of brightness and photostability and an increase up to 600% in the fluorescence response to calcium. In dissociated neurons, iGECI detects spontaneous neuronal activity and electrically and optogenetically induced firing. We validated the performance of iGECI up to a depth of almost 400 µm in acute brain slices using one-photon light-sheet imaging. Applying hybrid photoacoustic and fluorescence microscopy, we simultaneously monitored neuronal and hemodynamic activities in the mouse brain through an intact skull, with resolutions of ~3 μm (lateral) and ~25-50 μm (axial). Using two-photon imaging, we detected evoked and spontaneous neuronal activity in the mouse visual cortex, with fluorescence changes of up to 25%. iGECI allows biosensors and optogenetic actuators to be multiplexed without spectral crosstalk.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.