ArticleScientific reports2020
DNA metabarcoding to unravel plant species composition in selected herbal medicines on the National List of Essential Medicines (NLEM) of Thailand.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Establishment of ITS-Derived Species-Specific PCR Assay Method for Discriminating Herbal Medicine Descurainiae Semen from Its Commercial Adulterants.Plants (Basel, Switzerland) · 2025Article
- Advances in the Identification Methods of Food-Medicine Homologous Herbal Materials.Foods (Basel, Switzerland) · 2025Review
- DNA metabarcoding unveils authenticity and adulteration in commercial Chinese polyherbal preparations: Renshen Jianpi Wan as a critical case study.Frontiers in pharmacology · 2025Article
- From Species to Varieties: How Modern Sequencing Technologies Are Shaping Medicinal Plant Identification.Genes · 2024Review
- Existing status and future advancements of adulteration detection techniques in herbal products.Molecular biology reports · 2024Review
- A combined approach of DNA metabarcoding collectively enhances the detection efficiency of medicinal plants in single and polyherbal formulations.Frontiers in plant science · 2023Article
- Ethnobotanical Survey of Medicinal Plants Used in Breast Cancer Treatment by Traditional Health Practitioners in Central Uganda.Journal of multidisciplinary healthcare · 2023Article
- Social Marketing Strategy to Promote Traditional Thai Medicines during COVID-19: KAP and DoI Two-Step Theory Application Process.International journal of environmental research and public health · 2022Article
- A detailed workflow to develop QIIME2-formatted reference databases for taxonomic analysis of DNA metabarcoding data.BMC genomic data · 2022Article
- Advancements and future prospective of DNA barcodes in the herbal drug industry.Frontiers in pharmacology · 2022Review
- Differentiation of Mitragyna speciosa, a narcotic plant, from allied Mitragyna species using DNA barcoding-high-resolution melting (Bar-HRM) analysis.Scientific reports · 2021Article
- Biopiracy: Abolish Corporate Hijacking of Indigenous Medicinal Entities.TheScientificWorldJournal · 2021Review
- Medicinal Plants and Herbal Products From Brazil: How Can We Improve Quality?Frontiers in pharmacology · 2020Article
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Authors and funding
4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Traditional medicines are widely traded across the globe and have received considerable attention in the recent past, with expectations of heightened demand in the future. However, there are increasing global concerns over admixture, which can affect the quality, safety, and efficacy of herbal medicinal products. In this study, we aimed to use DNA metabarcoding to identify 39 Thai herbal products on the Thai National List of Essential Medicines (NLEM) and assess species composition and admixture. Among the products, 24 samples were in-house-prepared formulations, and 15 samples were registered formulations. In our study, DNA metabarcoding analysis using ITS2 and rbcL barcode regions were employed to identify herbal ingredients mentioned in the products. The nuclear region, ITS2, was able to identify herbal ingredients in the products at the genus- and family-levels in 55% and 63% of cases, respectively. The chloroplast gene, rbcL, enabled genus- and family-level identifications in 58% and 73% of cases, respectively. In addition, plant species were detected in larger numbers (Family identified, absolute %) in registered herbal products than in in-house-prepared formulations. The level of fidelity increases concerns about the reliability of the products. This study highlights that DNA metabarcoding is a useful analytical tool when combined with advanced chemical techniques for the identification of plant species in highly processed, multi-ingredient herbal products.
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