Visualization and quantification of dynamic intercellular coupling in human embryonic stem cells using single cell sonoporation.

Zhenzhen Fan, Xufeng Xue, Jianping Fu, Cheri X Deng

Abstract read

In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Rapid responses of human pluripotent stem cells to cyclic mechanical strains applied to integrin by acoustic tweezing cytometry.Scientific reports · 2023
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Zhenzhen FanDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.

Xufeng XueDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.

Jianping FuDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.

Cheri X DengDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA. cxdeng@umich.edu.

Funding

Acoustic tweezing cytometry: technology development and stem cell applicationsR01EB019436 · NIBIB · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DENG, CHERI X, FU, JIANPING · 2015 to 2018

$1.9M

NIBIB NIH HHS R01 EB019436

6 · The paper itself

Abstract

Gap junctions (GJs), which are proteinaceous channels, couple adjacent cells by permitting direct exchange of intracellular molecules with low molecular weights. GJ intercellular communication (GJIC) plays a critical role in regulating behaviors of human embryonic stem cells (hESCs), affecting their proliferation and differentiation. Here we report a novel use of sonoporation that enables single cell intracellular dye loading and dynamic visualization/quantification of GJIC in hESC colonies. By applying a short ultrasound pulse to excite single microbubbles tethered to cell membranes, a transient pore on the cell membrane (sonoporation) is generated which allows intracellular loading of dye molecules and influx of Ca

Indexed as

Cell CommunicationCell DifferentiationCell LineFluorescent DyesGap JunctionsHuman Embryonic Stem CellsHumansIntercellular JunctionsMicrobubblesSonicationFluorescent Dyes

Identifiers

PMID33106521

DOI10.1016/j.ceca.2016.02.011

PMCPMC7589565

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.