A New High-Throughput Screening Method to Detect Antimicrobial Volatiles from Metagenomic Clone Libraries.

Franz Stocker, Melanie M Obermeier, Katharina Resch, Gabriele Berg, Christina A Müller Bogotá

Abstract read

In one paragraph

Article in Antibiotics (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Franz StockerInstitute of Environmental Biotechnology, Graz University of Technology, Petersgasse 12/I, 8010 Graz, Austria.

Melanie M ObermeierInstitute of Environmental Biotechnology, Graz University of Technology, Petersgasse 12/I, 8010 Graz, Austria.ORCID 0000-0002-5450-0399

Katharina ReschInstitute of Environmental Biotechnology, Graz University of Technology, Petersgasse 12/I, 8010 Graz, Austria.

Gabriele BergInstitute of Environmental Biotechnology, Graz University of Technology, Petersgasse 12/I, 8010 Graz, Austria.

Christina A Müller BogotáInstitute of Environmental Biotechnology, Graz University of Technology, Petersgasse 12/I, 8010 Graz, Austria.ORCID 0000-0002-6590-7936

Funding

Österreichische Forschungsförderungsgesellschaft 24.081

6 · The paper itself

Abstract

The ever-growing spread of resistance in medicine and agriculture highlights the need to identify new antimicrobials. Microbial volatile organic compounds (VOCs) are one of the most promising groups of chemicals to meet this need. These rarely exploited molecules exhibit antimicrobial activity and their high vapour pressure makes them ideal for application in surface sterilisation, and in particular, in biofumigation. Therefore, we adapted the previously developed Two Clamp VOCs Assay (TCVA) to a new high-throughput screening for the detection of novel antifungal VOCs from metagenomic clone libraries. As a proof of concept, we tested the new high-throughput TCVA (htTCVA) by sourcing a moss metagenomic library against

Indexed as

antimicrobialsfunctional metagenomicsFusarium spp.high-throughput screeningSphagnumvolatiles

Identifiers

PMID33105853

DOI10.3390/antibiotics9110726

PMCPMC7690600

What OpenQuestion holds

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LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.