Evidence map›Paper›PMID 33103565›Full record

ArticleRNA biology2021

Introducing differential RNA-seq mapping to track the early infection phase for

Laura Wicke, Falk Ponath, Lucas Coppens, Milan Gerovac, Rob Lavigne, Jörg Vogel

Open access · bronzeAbstract read
In one paragraph

Article in RNA biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 38 citations in OpenAlex.

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  11. Review
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  15. Impact of phage predation onRNA biology · 2023
    Article
  16. Complete genome analysis ofFrontiers in cellular and infection microbiology · 2023
    Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Laura WickeInstitute for Molecular Infection Biology (IMIB), Medical Faculty, University of Würzburg, Würzburg, Germany.ORCID 0000-0001-7683-5328
Falk PonathHelmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), Würzburg, Germany.ORCID 0000-0002-2144-0545
Lucas CoppensDepartment of Biosystems, Laboratory of Gene Technology, KU Leuven, Leuven, Belgium.
Milan GerovacInstitute for Molecular Infection Biology (IMIB), Medical Faculty, University of Würzburg, Würzburg, Germany.ORCID 0000-0002-6929-7178
Rob LavigneDepartment of Biosystems, Laboratory of Gene Technology, KU Leuven, Leuven, Belgium.ORCID 0000-0001-7377-1314
Jörg VogelInstitute for Molecular Infection Biology (IMIB), Medical Faculty, University of Würzburg, Würzburg, Germany.ORCID 0000-0003-2220-1404
University of Würzburg · DEKU Leuven · BEHelmholtz Centre for Infection Research · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

As part of the ongoing renaissance of phage biology, more phage genomes are becoming available through DNA sequencing. However, our understanding of the transcriptome architecture that allows these genomes to be expressed during host infection is generally poor. Transcription start sites (TSSs) and operons have been mapped for very few phages, and an annotated global RNA map of a phage - alone or together with its infected host - is not available at all. Here, we applied differential RNA-seq (dRNA-seq) to study the early, host takeover phase of infection by assessing the transcriptome structure of

Indexed as

Gene Expression Regulation, BacterialGene Expression Regulation, ViralGenome, ViralAdhesins, BacterialAntibiosisBacterial Outer Membrane ProteinsBacterial ProteinsBiofilmsChromosome MappingDNA-Binding ProteinsGene OntologyMolecular Sequence AnnotationPseudomonas aeruginosaPseudomonas PhagesRNA, ViralSequence Analysis, RNAAdhesins, BacterialBacterial Outer Membrane ProteinsBacterial ProteinsCdrA protein, Pseudomonas aeruginosaDNA-Binding ProteinsparD protein, BacteriaRNA, ViralBacteriophage ɸKZdifferential expressiondRNA-seqphage-host interactionPseudomonas aeruginosatranscription start site

Identifiers

PMID33103565
PMCPMC8244752
OpenAlexW3094236352

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.