ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2020
Controlled Deposition of 3D Matrices to Direct Single Cell Functions.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Machine learning-driven single-cell phenotyping in size-controlled microenvironmentsLab on a chip · 2026Article
- FLIM quality metric visualization as a means to validate consistency across large-area non-homogeneous FLIM datasets.Methods and applications in fluorescence · 2026Article
- Innate Immune-Cloaked Microgel-Coated Mesenchymal Stromal Cells Reverse Persistent Pulmonary Fibrosis via Reparative Macrophages.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- The role of non-linear viscoelastic hydrogel mechanics in cell culture and transduction.Materials today. Bio · 2025Review
- FAK regulates tension transmission to the nucleus and endothelial transcriptome independent of kinase activity.Cell reports · 2024Article
- The microparticulate inks for bioprinting applications.Materials today. Bio · 2024Review
- Towards single cell encapsulation for precision biology and medicine.Advanced drug delivery reviews · 2023Review
- Recent advancements in single-cell metabolic analysis for pharmacological research.Journal of pharmaceutical analysis · 2023Review
- Mapping mechanical stress in curved epithelia of designed size and shape.Nature communications · 2023Article
- Cell-extracellular matrix mechanotransduction in 3D.Nature reviews. Molecular cell biology · 2023Review
- Massive and efficient encapsulation of single cells in monodisperse droplets and collagen-alginate microgels using a microfluidic device.Frontiers in bioengineering and biotechnology · 2023Article
- Article
- Deterministic Single Cell Encapsulation in Asymmetric Microenvironments to Direct Cell Polarity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Microfluidics Fabrication of Micrometer-Sized Hydrogels with Precisely Controlled Geometries for Biomedical Applications.Advanced healthcare materials · 2022Review
- Non-small cell lung carcinoma spheroid models in agarose microwells for drug response studies.Lab on a chip · 2022Article
- Matrix biophysical cues direct mesenchymal stromal cell functions in immunity.Acta biomaterialia · 2021Review
- Hydrogel Micropost Arrays with Single Post Tunability to Study Cell Volume and Mechanotransduction.Advanced biosystems · 2020Article
- Controlled Deposition of 3D Matrices to Direct Single Cell Functions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Advances in engineered hydrogels reveal how cells sense and respond to 3D biophysical cues. However, most studies rely on interfacing a population of cells in a tissue-scale bulk hydrogel, an approach that overlooks the heterogeneity of local matrix deposition around individual cells. A droplet microfluidic technique to deposit a defined amount of 3D hydrogel matrices around single cells independently of material composition, elasticity, and stress relaxation times is developed. Mesenchymal stem cells (MSCs) undergo isotropic volume expansion more rapidly in thinner gels that present an Arg-Gly-Asp integrin ligand. Mathematical modeling and experiments show that MSCs experience higher membrane tension as they expand in thinner gels. Furthermore, thinner gels facilitate osteogenic differentiation of MSCs. By modulating ion channels, it is shown that isotropic volume expansion of single cells predicts intracellular tension and stem cell fate. The results suggest the utility of precise microscale gel deposition to control single cell functions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.