ArticleJournal of biomolecular techniques : JBT2020
Got Glycogen?: Development and Multispecies Validation of the Novel Preserve, Precipitate, Lyse, Precipitate, Purify (PPLPP) Workflow for Environmental DNA Extraction from Longmire's Preserved Water Samples.
Article in Journal of biomolecular techniques : JBT, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 28 citations in OpenAlex.
- Environmental DNA as a tool to reconstruct catch composition for longline fisheries vessels.Scientific reports · 2024Article
- Detection of the Endangered Siamese Bat Catfish (Animals : an open access journal from MDPI · 2023Article
- Environmental DNA analysis confirms extant populations of the cryptic Irwin's turtle within its historical range.BMC ecology and evolution · 2022Article
- Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
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Abstract
Unfiltered and filtered water samples can be used to collect environmental DNA (eDNA). We developed the novel "Preserve, Precipitate, Lyse, Precipitate, Purify" (PPLPP) workflow to efficiently extract eDNA from Longmire's preserved unfiltered and filtered water samples (44-100% recovery). The PPLPP workflow includes initial glycogen-aided isopropanol precipitation, guanidium hypochlorite and Triton X-100-based lysis, terminal glycogen-aided polyethylene glycol precipitation, and inhibitor purification. Three novel eDNA assays that exclusively target species invasive to Australia were also developed: Tilapia_v2_16S concurrently targets
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Registered trials
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