ArticleLetters in applied microbiology2021
Impact of pH on citric acid antimicrobial activity against Gram-negative bacteria.
Article in Letters in applied microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
43 citing papers in PubMed, 1 synthesis or guideline pooled it, 112 citations in OpenAlex.
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- The role of citric acid in denture cleansing: Effects on biofilm reduction and corrosion resistance of Co-Cr alloys.Brazilian oral research · 2025Article
- Non-enveloped virus inactivation potency of sodium dodecyl sulfate with citric and glutamic acids.Frontiers in microbiology · 2025Article
- The Results of Intestinal Villi of Laying Hens Exposed With Avian PathogenicVeterinary medicine international · 2025Article
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Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The antimicrobial activity of citric acid (CA) is often evaluated without pH adjustment or control and its impact on micro-organisms is better understood in acidic conditions. However, the biocidal action of the fully ionized CA molecule, predominantly available at higher pH, has not been previously investigated. The objective of this study was to evaluate the antimicrobial effect of high (10%) and low (1%) concentrations of CA, each adjusted over a wide range of pH values (4·5, 6·5 and 9·5) relative to the controls exposed to corresponding pH levels alone (no CA). The viability and morphology of Escherichia coli and Klebsiella aerogenes were evaluated using a culture-based enumeration assay in parallel with direct SEM imaging. Overall, the highest membrane damage and loss in viability were achieved with 10% CA at pH 9·5, which yielded at least 4·6 log
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Registered trials
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