ArticleNucleic acids research1987
The UAS of the yeast PGK gene contains functionally distinct domains.
Article in Nucleic acids research, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 50 citations in OpenAlex.
- Yeast silencers can act as orientation-dependent gene inactivation centers that respond to environmental signals.Molecular and cellular biology · 1995Article
- Expression of an endogenous and a heterologous gene in Candida maltosa by using a promoter of a newly-isolated phosphoglycerate kinase (PGK) gene.Current genetics · 1994Article
- Yeast intragenic transcriptional control: activation and repression sites within the coding region of the Saccharomyces cerevisiae LPD1 gene.Molecular and cellular biology · 1994Article
- A regulatory element in the CHA1 promoter which confers inducibility by serine and threonine on Saccharomyces cerevisiae genes.Molecular and cellular biology · 1993Article
- A complex regulatory element from the yeast gene ENO2 modulates GCR1-dependent transcriptional activation.Molecular and cellular biology · 1993Article
- Global regulators of chromosome function in yeast.Antonie van Leeuwenhoek · 1992Review
- The role of promoter elements of a ribosomal protein gene in Saccharomyces cerevisiae under various physiological conditions.Molecular & general genetics : MGG · 1992Article
- Characterization of the DNA-binding activity of GCR1: in vivo evidence for two GCR1-binding sites in the upstream activating sequence of TPI of Saccharomyces cerevisiae.Molecular and cellular biology · 1992Article
- A phylogenetic analysis based on the gene encoding phosphoglycerate kinase.Journal of molecular evolution · 1992Article
- A multi-component upstream activation sequence of the Saccharomyces cerevisiae glyceraldehyde-3-phosphate dehydrogenase gene promoter.Molecular & general genetics : MGG · 1991Article
- Phosphorylation influences the binding of the yeast RAP1 protein to the upstream activating sequence of the PGK gene.Nucleic acids research · 1990Article
- The DNA-binding protein RAP1 is required for efficient transcriptional activation of the yeast PYK glycolytic gene.Current genetics · 1990Article
- ARS binding factor 1 binds adjacent to RAP1 at the UASs of the yeast glycolytic genes PGK and PYK1.Nucleic acids research · 1990Article
- TUF factor binds to the upstream region of the pyruvate decarboxylase structural gene (PDC1) of Saccharomyces cerevisiae.Molecular & general genetics : MGG · 1990Article
- Multiple factors bind the upstream activation sites of the yeast enolase genes ENO1 and ENO2: ABFI protein, like repressor activator protein RAP1, binds cis-acting sequences which modulate repression or activation of transcription.Molecular and cellular biology · 1990Article
- Characterisation of the DNA binding domain of the yeast RAP1 protein.Nucleic acids research · 1990Article
- Efficient transcription of the glycolytic gene ADH1 and three translational component genes requires the GCR1 product, which can act through TUF/GRF/RAP binding sites.Molecular and cellular biology · 1990Article
- Transcriptional control of the Saccharomyces cerevisiae PGK gene by RAP1.Molecular and cellular biology · 1989Article
- Characterization of the transcriptional potency of sub-elements of the UAS of the yeast PGK gene in a PGK mini-promoter.Nucleic acids research · 1989Article
- An ARS/silencer binding factor also activates two ribosomal protein genes in yeast.Nucleic acids research · 1989Article
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5 authors at 1 institution in 1 country.
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Abstract
The upstream activation site (UAS) of the yeast phosphoglycerate kinase gene (PGK) has been localised by deletion analysis (1). Here we show that the UASPGK contains two functionally distinct domains. These two domains, designated activator (A) and modulator (M), appear to be located within bases -460 to -402 and -531 to -461, respectively, relative to the initiating ATG; although it is possible that part of the M domain resides within the A domain. They have been shown, using a heterologous assay promoter, to have distinct transcriptional functions. Domain A is responsible for activation of transcription whilst domain M is required for carbon source dependent regulation of transcription. Protein-DNA binding studies have demonstrated that the DNA fragment containing domain M has high affinity for at least one specific DNA-binding protein, whilst domain A does not appear to interact strongly in protein-binding assays under the same conditions. The domain M binding activity is dependent on the carbon source in the growth medium and may be functional in the carbon source control of PGK expression.
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