ArticleJournal of biomedical optics2020
High-speed axially swept light sheet microscopy using a linear MEMS phased array for isotropic resolution.
Article in Journal of biomedical optics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- Practical considerations for quantitative light sheet fluorescence microscopy.Nature methods · 2022Review
- Isotropic imaging across spatial scales with axially swept light-sheet microscopy.Nature protocols · 2022Review
- High contrast, isotropic, and uniform 3D-imaging of centimeter-scale scattering samples using structured illumination light-sheet microscopy with axial sweeping.Biomedical optics express · 2022Article
- Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm.International journal of molecular sciences · 2022Review
- CompassLSM: axially swept light-sheet microscopy made simple.Biomedical optics express · 2021Article
- Tunable Wide-Field Illumination and Single-Molecule Photoswitching with a Single MEMS Mirror.ACS photonics · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
significanceAxially swept light sheet microscopy is used for deconvolution-free, high-resolution 3D imaging, but usually the axial scan mechanism reduces the top imaging speed. Phased arrays (PAs) for axial scanning enable both high resolution and high speed.
aimA high-speed PA with an update rate faster than the camera row read time is used to track the rolling shutter at camera-limited rates. APPROACH: The point spread function is evaluated to ensure sub-micron isotropic resolution, and the technique is demonstrated on a live Drosophila embryo.
resultsIsotropic resolution is shown down to 720 ± 55 nm in all three spatial dimensions. With an update rate of 2.85 μs, the PA tracks the camera sensor rolling shutter at camera-limited rates. Features in the Drosophila embryo are resolved clearly compared with the equivalent static light sheet case. The random-access nature of the PA enables a camera sensor readout in the same direction for each frame to maintain even temporal sampling in image sequences with no speed loss.
conclusionsUse of PAs is compatible with axially swept light sheet microscopy and offers significant improvements in speed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.