ReviewNature protocols2020
Tutorial: design and fabrication of nanoparticle-based lateral-flow immunoassays.
Review in Nature protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 175 papers.
What it found
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The trial behind it
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Who cites it
175 citing papers in PubMed.
- A rapid detection of Nipah virus nucleocapsid antigen in inactivated viral cultures.Emerging microbes & infections · 2026Article
- Development and application of a smartphone-based thermal contrast amplification reader for photothermal lateral flow immunoassays.Microsystems & nanoengineering · 2026Article
- Overcoming sensitivity limitations in lateral flow immunoassays for small-molecule detection: from tracer engineering to assay format innovation.Analytical and bioanalytical chemistry · 2026Review
- Antibody-based sensing of pesticide residues and mycotoxins in plant-derived foods: A narrative review of ELISA, lateral-flow immunoassays and immunosensors.Food chemistry: X · 2026Review
- Analysis of multiplexed SERS-based lateral flow assays: turning qualitative into quantitative for inflammatory bowel disease monitoring.The Analyst · 2026Article
- Coordination-Driven Metal-Phenolic Network Complexes for Programmable Colorimetric Signal Transduction and Defined Nano-Bio Interfaces.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Yes/No Quantitative Analysis of Single-Stranded Oligonucleotides with Lateral Flow Assays.Biosensors · 2026Article
- Dual-readout lateral flow immunoassay based on upconversion nanoparticle-gold nanoparticle luminescence resonance energy transfer for rapid determination of 17β-estradiol.Mikrochimica acta · 2026Article
- Electrochemical lateral flow assay with ELISA-level performance for detecting plant diseases in East Africa.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Self-Contained Lateral-Flow Microfluidic Bead-Based Assay for Rapid Quantification of Early-Stage Kidney Biomarkers.Analytical chemistry · 2026Article
- A Rational Optimization Approach for the Development of a Multiplexed Lateral Flow Immunoassay: Detection of Nonepithelial Ovarian Cancer Markers in Human Serum.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Article
- Evolution of Next-Generation Multiplex Lateral Flow Immunoassays: From Engineered Nanomaterials to AI-Driven Detection.Biosensors · 2026Review
- Integrated triplex LFIA platform for decentralized molecular subtyping of breast cancer.RSC advances · 2026Article
- Controlled immobilization of single-domain antibodies using cellulose-binding modules for ABRSC advances · 2026Article
- Fishnet-like 2D magnetic nanozyme probe enables ultrasensitive bedside immunodiagnosis of bone infection pathogens.Bioactive materials · 2026Article
- Label matters: comparing gold nanoparticles and nanoshells with upconversion nanoparticles for quantitative lateral flow immunoassays.Mikrochimica acta · 2026Article
- Nanoparticle-based biosensor integrated with CRISPR/Cas12b platform for sensitive and visual identification of hepatitis B virus pregenomic RNA in chronic hepatitis B patients.BMC microbiology · 2026Article
- The evolution of next-generation lateral flow assays for bacterial and fungal diagnostics.Mikrochimica acta · 2026Review
- Alkyne Nitro Tag Enables Stable and Efficient Protein Functionalization of Gold Nanoparticles.ACS applied materials & interfaces · 2026Article
115 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lateral-flow assays (LFAs) are quick, simple and cheap assays to analyze various samples at the point of care or in the field, making them one of the most widespread biosensors currently available. They have been successfully employed for the detection of a myriad of different targets (ranging from atoms up to whole cells) in all type of samples (including water, blood, foodstuff and environmental samples). Their operation relies on the capillary flow of the sample throughout a series of sequential pads, each with different functionalities aiming to generate a signal to indicate the absence/presence (and, in some cases, the concentration) of the analyte of interest. To have a user-friendly operation, their development requires the optimization of multiple, interconnected parameters that may overwhelm new developers. In this tutorial, we provide the readers with: (i) the basic knowledge to understand the principles governing an LFA and to take informed decisions during lateral flow strip design and fabrication, (ii) a roadmap for optimal LFA development independent of the specific application, (iii) a step-by-step example procedure for the assembly and operation of an LF strip for the detection of human IgG and (iv) an extensive troubleshooting section addressing the most frequent issues in designing, assembling and using LFAs. By changing only the receptors, the provided example procedure can easily be adapted for cost-efficient detection of a broad variety of targets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.