Evidence map›Paper›PMID 33097925›Full record

ArticleNature protocols2020

Global analysis of repetitive DNA from unassembled sequence reads using RepeatExplorer2.

Petr Novák, Pavel Neumann, Jiří Macas

Abstract read
PubMed Publisher
In one paragraph

Article in Nature protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 191 papers.

0numbers the graph read from it
0cells of the map it votes in
191citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

191 citing papers in PubMed.

  1. Article
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  4. Satellite DNAs in Drosophila koepferae (repleta group) reveal patterns of origin, chromosomal organization, transcription, and turnover in the buzzatii cluster.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026
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  9. A single species, different repeatomes? Genomic plasticity in Passiflora foetida L. complex and comparative insights across the subgenus Passiflora.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026
    Article
  10. Article
  11. Satellite DNA evolution in two holocentric species of Edessa true bugs (Hemiptera: Pentatomidae) with unusually high heterochromatin abundance.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026
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  16. Nuclear genome profiling of two species ofNAR genomics and bioinformatics · 2026
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  18. Cytogenomics of Myloplus tiete reveals conserved satellite DNAs since the Late Eocene in Serrasalmidae (Teleostei, Characiformes).Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026
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  19. Article
  20. Article

131 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Petr NovákBiology Centre, Czech Academy of Sciences, Institute of Plant Molecular Biology, České Budějovice, Czech Republic.ORCID http://orcid.org/0000-0002-5068-9681
Pavel NeumannBiology Centre, Czech Academy of Sciences, Institute of Plant Molecular Biology, České Budějovice, Czech Republic.ORCID http://orcid.org/0000-0001-6711-6639
Jiří MacasBiology Centre, Czech Academy of Sciences, Institute of Plant Molecular Biology, České Budějovice, Czech Republic. macas@umbr.cas.cz.ORCID http://orcid.org/0000-0003-0829-1570

Funding

Ministerstvo Školství, Mládeže a Tělovýchovy (Ministry of Education, Youth and Sports) CZ.02.1.01/0.0/0.0/16_013/0001777Ministerstvo Školství, Mládeže a Tělovýchovy (Ministry of Education, Youth and Sports) LM2015047
6 · The paper itself

Abstract

RepeatExplorer2 is a novel version of a computational pipeline that uses graph-based clustering of next-generation sequencing reads for characterization of repetitive DNA in eukaryotes. The clustering algorithm facilitates repeat identification in any genome by using relatively small quantities of short sequence reads, and additional tools within the pipeline perform automatic annotation and quantification of the identified repeats. The pipeline is integrated into the Galaxy platform, which provides a user-friendly web interface for script execution and documentation of the results. Compared to the original version of the pipeline, RepeatExplorer2 provides automated annotation of transposable elements, identification of tandem repeats and enhanced visualization of analysis results. Here, we present an overview of the RepeatExplorer2 workflow and provide procedures for its application to (i) de novo repeat identification in a single species, (ii) comparative repeat analysis in a set of species, (iii) development of satellite DNA probes for cytogenetic experiments and (iv) identification of centromeric repeats based on ChIP-seq data. Each procedure takes approximately 2 d to complete. RepeatExplorer2 is available at https://repeatexplorer-elixir.cerit-sc.cz .

Indexed as

AnimalsCluster AnalysisDNADNA ProbesDNA Transposable ElementsGenomicsHigh-Throughput Nucleotide SequencingHumansRepetitive Sequences, Nucleic AcidSequence Analysis, DNASoftwareDNADNA ProbesDNA Transposable Elements

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.