ArticleScientific reports2020
Skin swabbing is a refined technique to collect DNA from model fish species.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 32 citations in OpenAlex.
- Protocol for non-invasive genotyping of axolotls using skin swabbing.STAR protocols · 2026Article
- Rapid CRISPR-Cas13a genetic identification enables new opportunities for listed Chinook salmon management.Molecular ecology resources · 2025Article
- Validation of two swabbing methods to sample DNA for genotyping Atlantic bluefin tuna (Thunnus thynnus).Scientific reports · 2025Article
- Comparison of whole genome sequencing performance from fish swabs and fin clips.BMC research notes · 2025Article
- A highly cost-effective, eco-friendly tissue lysis and extraction method for faster DNA isolation from fish fin.PloS one · 2025Article
- Pain management in zebrafish : Report from a FELASA Working Group.Laboratory animals · 2024Review
- Genetic identification and reiterated captures suggest that theZoological research · 2023Article
- Non-Lethal Detection ofViruses · 2023Article
- A Rapid and Noninvasive Method That Extracts Polymerase Chain Reaction-Ready Genomic DNA from Adult Zebrafish.Zebrafish · 2022Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
Abstract
Model fish species such as sticklebacks and zebrafish are frequently used in studies that require DNA to be collected from live animals. This is typically achieved by fin clipping, a procedure that is simple and reliable to perform but that can harm fish. An alternative procedure to sample DNA involves swabbing the skin to collect mucus and epithelial cells. Although swabbing appears to be less invasive than fin clipping, it still requires fish to be netted, held in air and handled-procedures that can cause stress. In this study we combine behavioural and physiological analyses to investigate changes in gene expression, behaviour and welfare after fin clipping and swabbing. Swabbing led to a smaller change in cortisol release and behaviour on the first day of analysis compared to fin clipping. It also led to less variability in data suggesting that fewer animals need to be measured after using this technique. However, swabbing triggered some longer term changes in zebrafish behaviour suggesting a delayed response to sample collection. Skin swabbing does not require the use of anaesthetics and triggers fewer changes in behaviour and physiology than fin clipping. It is therefore a more refined technique for DNA collection with the potential to improve fish health and welfare.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.