Evidence map›Paper›PMID 33096877›Full record

ReviewPolymers2020

Circular Intensity Differential Scattering for Label-Free Chromatin Characterization: A Review for Optical Microscopy.

Aymeric Le Gratiet, Riccardo Marongiu, Alberto Diaspro

Abstract readReview
In one paragraph

Review in Polymers, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Aymeric Le GratietNanoscopy and NIC@IIT, Istituto Italiano di Tecnologia, Via Enrico Melen 83, 16152 Genova, Italy.ORCID 0000-0002-4281-7065
Riccardo MarongiuNanoscopy and NIC@IIT, Istituto Italiano di Tecnologia, Via Enrico Melen 83, 16152 Genova, Italy.
Alberto DiasproNanoscopy and NIC@IIT, Istituto Italiano di Tecnologia, Via Enrico Melen 83, 16152 Genova, Italy.ORCID 0000-0002-4916-5928

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Circular Intensity Differential Scattering (CIDS) provides a differential measurement of the circular right and left polarized light and has been proven to be a gold standard label-free technique to study the molecular conformation of complex biopolymers, such as chromatin. In early works, it has been shown that the scattering component of the CIDS signal gives information from the long-range chiral organization on a scale down to 1/10th-1/20th of the excitation wavelength, leading to information related to the structure and orientation of biopolymers in situ at the nanoscale. In this paper, we review the typical methods and technologies employed for measuring this signal coming from complex macro-molecules ordering. Additionally, we include a general description of the experimental architectures employed for spectroscopic CIDS measurements, angular or spectral, and of the most recent advances in the field of optical imaging microscopy, allowing a visualization of the chromatin organization in situ.

Indexed as

biopolymersDNA organizationmicroscopypolarization

Identifiers

PMID33096877
PMCPMC7588990

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.