Evidence map›Paper›PMID 33096541›Full record

ArticlePediatric research2021

The effects of IGF-1 and erythropoietin on apoptosis and telomerase activity in necrotizing enterocolitis model.

Mete Akisu, Burak Durmaz, Ozge Altun Koroglu, Sema Unlubay, Mehmet Yalaz, Haluk Akin, Utku Ates, Meral Baka, Ferda Ozkinay, Ozgur Cogulu and 1 more

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Article in Pediatric research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it, 7 citations in OpenAlex.

  1. Pooled it
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  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Mete AkisuDivision of Neonatology, Department of Pediatrics, Faculty of Medicine, Ege University, Izmir, Turkey.
Burak DurmazDepartment of Medical Genetics, Faculty of Medicine, Ege University, Izmir, Turkey. burak.durmaz@ege.edu.tr.
Ozge Altun KorogluDivision of Neonatology, Department of Pediatrics, Faculty of Medicine, Ege University, Izmir, Turkey.
Sema UnlubayDivision of Genetics and Teratology, Department of Pediatrics, Faculty of Medicine, Ege University, Izmir, Turkey.
Mehmet YalazDivision of Neonatology, Department of Pediatrics, Faculty of Medicine, Ege University, Izmir, Turkey.
Haluk AkinDepartment of Medical Genetics, Faculty of Medicine, Ege University, Izmir, Turkey.
Utku AtesDepartment of Histology and Embryology, Faculty of Medicine, Ege University, Izmir, Turkey.
Meral BakaDepartment of Histology and Embryology, Faculty of Medicine, Ege University, Izmir, Turkey.
Ferda OzkinayDepartment of Medical Genetics, Faculty of Medicine, Ege University, Izmir, Turkey.
Ozgur CoguluDepartment of Medical Genetics, Faculty of Medicine, Ege University, Izmir, Turkey.
Nilgun KultursayDivision of Neonatology, Department of Pediatrics, Faculty of Medicine, Ege University, Izmir, Turkey.
Ege University · TR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundApoptosis that occurs after hypoxia/reoxygenation (H/R) has an important role in the pathogenesis of necrotizing enterocolitis (NEC). Telomerase activity, showing the regeneration capacity, may also be important in the recovery process. Therefore, we aimed to investigate the effects of insulin-like growth factor-1 (IGF-1) and erythropoietin (EPO) on apoptosis and telomerase activity in an H/R model.

methodsYoung mice were divided into four groups each containing ten Balb/c mice. Group 1 (H/R) were exposed to H/R; group 2 and group 3 were pretreated with IGF-1 and EPO, respectively, for 7 days before H/R. Group 4 served as control. Intestinal injury was evaluated by histological scoring and assessment of apoptosis was performed by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) test. Proapoptotic and antiapoptotic gene expressions and telomerase activity were analyzed by real-time PCR.

resultsIGF-1- and EPO-treated animals had decreased histological damage and apoptosis, confirmed by TUNEL test and caspase activity. Telomerase activity was increased in these animals in addition to increased expression of antiapoptotic genes. However, proapoptotic gene expressions were not statistically different.

conclusionsThe protective effects of IGF-1 and EPO in H/R damage may be through increased expression of antiapoptotic genes and increased telomerase activity, especially for IGF-1. IMPACT: This is a comprehensive study measuring various variables, namely IGF-1, EPO, apoptosis, apoptotic and antiapoptotic genes, and telomerase activity in the NEC model. The intestinal protective effects of IGF-1 and EPO in H/R damage may occur through increased expression of antiapoptotic genes and increased telomerase activity. To the best of our knowledge, telomerase activity has not been investigated in the NEC model before. Regarding our results, novel strategies may be implemented for the early definitive diagnosis, robust preventive measures, and effective treatment modalities for NEC.

Indexed as

AnimalsApoptosisDisease Models, AnimalEnterocolitis, NecrotizingErythropoietinInsulin-Like Growth Factor IMiceMice, Inbred BALB CTelomeraseErythropoietininsulin-like growth factor-1, mouseInsulin-Like Growth Factor ITelomerase

Identifiers

PMID33096541
OpenAlexW3094347409

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.