ArticleStructure (London, England : 1993)2021
Serial cryoFIB/SEM Reveals Cytoarchitectural Disruptions in Leigh Syndrome Patient Cells.
Article in Structure (London, England : 1993), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed.
- Cryoelectron tomography of HIV-1 cell-cell transmission conjugates reveals a secluded environment for viral assembly and transfer.bioRxiv : the preprint server for biology · 2026Article
- Preparation of Vitrified Mammalian Cells for In Situ Cryo-Electron Tomography.Current protocols · 2025Article
- Reduction of SEM charging artefacts in native cryogenic biological samples.Nature communications · 2025Article
- A new age in structural S-layer biology: Experimental and in silico milestones.The Journal of biological chemistry · 2025Review
- Zooming in and out: Exploring RNA Viral Infections with Multiscale Microscopic Methods.Viruses · 2024Review
- Disruption of the mitochondrial network in a mouse model of Huntington's disease visualized by in-tissue multiscale 3D electron microscopy.Acta neuropathologica communications · 2024Article
- Cryo-electron tomography to study viral infection.Biochemical Society transactions · 2023Review
- Volume EM: a quiet revolution takes shape.Nature methods · 2023Article
- Precise targeting for 3D cryo-correlative light and electron microscopy volume imaging of tissues using a FinderTOP.Communications biology · 2023Article
- Article
- Ot2Rec: A semi-automatic, extensible, multi-software tomographic reconstruction workflow.Biological imaging · 2023Article
- Okapi-EM: A napari plugin for processing and analyzing cryogenic serial focused ion beam/scanning electron microscopy images.Biological imaging · 2023Article
- Structure and activity of particulate methane monooxygenase arrays in methanotrophs.Nature communications · 2022Article
- Volume electron microscopy.Nature reviews. Methods primers · 2022Article
- Super-resolution confocal cryo-CLEM with cryo-FIB milling forCurrent research in structural biology · 2022Article
- Review
- Article
- CorRelator: Interactive software for real-time high precision cryo-correlative light and electron microscopy.Journal of structural biology · 2021Article
- Correlative Multi-scale Cryo-imaging Unveils SARS-CoV-2 Assembly and Egress.Research square · 2021Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
The advancement of serial cryoFIB/SEM offers an opportunity to study large volumes of near-native, fully hydrated frozen cells and tissues at voxel sizes of 10 nm and below. We explored this capability for pathologic characterization of vitrified human patient cells by developing and optimizing a serial cryoFIB/SEM volume imaging workflow. We demonstrate profound disruption of subcellular architecture in primary fibroblasts from a Leigh syndrome patient harboring a disease-causing mutation in USMG5 protein responsible for impaired mitochondrial energy production.
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Registered trials
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