Evidence map›Paper›PMID 33095977›Full record

ArticleFEBS open bio2022

Biochemical and in silico identification of the active site and the catalytic mechanism of the circadian deadenylase HESPERIN.

Rafailia A A Beta, Athanasios Kyritsis, Veroniki Douka, Eirini Papanastasi, Marianna Rizouli, Demetres D Leonidas, Dimitrios Vlachakis, Nikolaos A A Balatsos

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS open bio, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 100% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Rafailia A A BetaDepartment of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.ORCID 0000-0002-3807-2224
Athanasios KyritsisDepartment of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
Veroniki DoukaDepartment of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
Eirini PapanastasiDepartment of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
Marianna RizouliDepartment of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
Demetres D LeonidasDepartment of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.ORCID 0000-0002-3874-2523
Dimitrios VlachakisGenetics Laboratory, Department of Biotechnology, Agricultural University of Athens, Greece.
Nikolaos A A BalatsosDepartment of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.ORCID 0000-0002-1146-1174
University of Thessaly · GRAgricultural University of Athens · GR

Funding

European Regional Development FundNational Research FoundationOMIC-ENGINE (MIS 5002636) implemented under "Reinforcement of the Research and Innovation Infrastructure", Operational Programme "Competitiveness, Entrepreneurship and Innovation" (NSRF 2014-2020) MIS 5002636State Scholarships Foundation (IKY) Fellowship of Excellence for Postgraduate Studies in Greece - Siemens Programme (to AK)University of Thessaly Research Committee 3439University of Thessaly Research Committee 3817University of Thessaly Research Committee 5851
6 · The paper itself

Abstract

The 24-h molecular clock is based on the stability of rhythmically expressed transcripts. The shortening of the poly(A) tail of mRNAs is often the first and rate-limiting step that determines the lifespan of a mRNA and is catalyzed by deadenylases. Herein, we determine the catalytic site of Hesperin, a recently described circadian deadenylase in plants, using a modified site-directed mutagenesis protocol and a custom vector, pATHRA. To explore the catalytic efficiency of AtHESPERIN, we investigated the effect of AMP and neomycin, and used molecular modeling simulations to propose a catalytic mechanism. Collectively, the biochemical and in silico results classify AtHESPERIN in the exonuclease-endonuclease-phosphatase deadenylase superfamily and contribute to the understanding of the intricate mechanisms of circadian mRNA turnover.

Indexed as

Catalytic DomainCatalysisRNA, MessengerRNA, MessengerAtHESPERINcircadian rhythmsdeadenylationmRNA decaypoly(A) tail

Identifiers

PMID33095977
PMCPMC9063446
OpenAlexW3094599197

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.