ArticleChemistry (Weinheim an der Bergstrasse, Germany)2021
Photoactivatable Fluorophore for Stimulated Emission Depletion (STED) Microscopy and Bioconjugation Technique for Hydrophobic Labels.
Article in Chemistry (Weinheim an der Bergstrasse, Germany), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 42 citations in OpenAlex.
- Phenoxazines with a PhototransferableJournal of the American Chemical Society · 2026Article
- Wash-Free Multi-Target Super-Resolution Microscopy With Photocaged DNA Labels.Angewandte Chemie (International ed. in English) · 2026Article
- Article
- On-Target Photoassembly of Pyronin Dyes for Super-Resolution Microscopy.Angewandte Chemie (International ed. in English) · 2025Article
- Bleaching protection and axial sectioning in fluorescence nanoscopy through two-photon activation at 515 nm.Nature communications · 2024Article
- Fluorescent fatty acid conjugates for live cell imaging of peroxisomes.Nature communications · 2024Article
- No-wash fluorogenic labeling of proteins for reversible photoswitching in live cells.Chemical science · 2024Article
- Review
- Photochemical Mechanisms of Fluorophores Employed in Single-Molecule Localization Microscopy.Angewandte Chemie (International ed. in English) · 2023Review
- Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes.Light, science & applications · 2022Article
- A multicomponent reaction platform towards multimodal near-infrared BODIPY dyes for STED and fluorescence lifetime imaging.RSC chemical biology · 2022Article
- A general design of caging-group-free photoactivatable fluorophores for live-cell nanoscopy.Nature chemistry · 2022Article
- Bis-Rhodamines Bridged with a Diazoketone Linker: Synthesis, Structure, and Photolysis.The Journal of organic chemistry · 2022Article
- Photoactivatable Fluorescent Dyes with Hydrophilic Caging Groups and Their Use in Multicolor Nanoscopy.Journal of the American Chemical Society · 2021Article
- MINSTED fluorescence localization and nanoscopy.Nature photonics · 2021Article
- GlycoBODIPYs: Sugars Serving as a Natural Stock for Water-soluble Fluorescent Probes of Complex Chiral Morphology.Angewandte Chemie (International ed. in English) · 2021Article
- Photoactivatable Fluorophore for Stimulated Emission Depletion (STED) Microscopy and Bioconjugation Technique for Hydrophobic Labels.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
The use of photoactivatable dyes in STED microscopy has so far been limited by two-photon activation through the STED beam and by the fact that photoactivatable dyes are poorly solvable in water. Herein, we report ONB-2SiR, a fluorophore that can be both photoactivated in the UV and specifically de-excited by STED at 775 nm. Likewise, we introduce a conjugation and purification protocol to effectively label primary and secondary antibodies with moderately water-soluble dyes. Greatly reducing dye aggregation, our technique provides a defined and tunable degree of labeling, and improves the imaging performance of dye conjugates in general.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.