Evidence map›Paper›PMID 33095410›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2021

First detection of canine parvovirus 2b DNA in a crab-eating fox pup (Cerdocyon thous, Linnaeus, 1766).

Caroline Giuseppa Spera, Fernanda Louise Pereira Lavorente, Elis Lorenzetti, Gustavo de Calasans Marques, Luana de Almeida Freitas, Maíra Bonamin Martins, Carlos Roberto Teixeira, Amauri Alcindo Alfieri, Alice Fernandes Alfieri

Abstract readCase Reports
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Caroline Giuseppa SperaLaboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Celso Garcia Cid Road, PR455 Km 380, P.O. Box 10011, Londrina, Paraná, 86057-970, Brazil.
Fernanda Louise Pereira LavorenteLaboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Celso Garcia Cid Road, PR455 Km 380, P.O. Box 10011, Londrina, Paraná, 86057-970, Brazil.
Elis LorenzettiLaboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Celso Garcia Cid Road, PR455 Km 380, P.O. Box 10011, Londrina, Paraná, 86057-970, Brazil.ORCID https://orcid.org/0000-0002-5032-712X
Gustavo de Calasans MarquesCenter for Medicine and Research of Wild Animals, Veterinary Hospital, Universidade Estadual Paulista - Julio de Mesquita Filho, P.O. Box 560, Botucatu, São Paulo, 18618-000, Brazil.
Luana de Almeida FreitasLaboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Celso Garcia Cid Road, PR455 Km 380, P.O. Box 10011, Londrina, Paraná, 86057-970, Brazil.
Maíra Bonamin MartinsAnimal Pathology, Veterinary Hospital, Universidade Estadual Paulista - Julio de Mesquita Filho, P.O. Box 560, Botucatu, São Paulo, 18618-000, Brazil.
Carlos Roberto TeixeiraCenter for Medicine and Research of Wild Animals, Veterinary Hospital, Universidade Estadual Paulista - Julio de Mesquita Filho, P.O. Box 560, Botucatu, São Paulo, 18618-000, Brazil.
Amauri Alcindo AlfieriLaboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Celso Garcia Cid Road, PR455 Km 380, P.O. Box 10011, Londrina, Paraná, 86057-970, Brazil. alfieri@uel.br.ORCID https://orcid.org/0000-0002-7024-4487
Alice Fernandes AlfieriLaboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Celso Garcia Cid Road, PR455 Km 380, P.O. Box 10011, Londrina, Paraná, 86057-970, Brazil.ORCID https://orcid.org/0000-0002-8607-956X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The crab-eating fox (Cerdocyon thous) is a small wild mammal present in all Brazilian biomes and in some countries of South America. This study aimed to verify the involvement of viral infectious agents in the death of a wild crab-eating fox pup (Cerdocyon thous) in Brazil. The Center for Medicine and Research of Wild Animals of the Universidade Estadual Paulista received a free-living crab-eating fox aged approximately 21 days and apparently healthy. After 13 days, the animal presented anorexia, diarrhea, fever, prostration, and neurological signs progressing to death with an inconclusive diagnosis. In a retrospective study, tissue fragments stored at - 80 °C were used to identify nucleic acids from major canine viruses, such as canine parvovirus-2 (CPV-2), canine adenovirus A types 1 and 2, canid alphaherpesvirus 1, and canine distemper virus. The amplified product with the expected length for CPV-2 was obtained from the heart fragment. After performing nucleotide (nt) sequencing of the amplicon, it was possible to demonstrate that the crab-eating fox strain exhibited high (99.8%) nt identity with the CPV-2b prototype (CPV-39 strain). Additionally, deduced amino acid (aa) sequence analysis showed the GAT codon for the aa Asp (D) at position 426 of the CPV-2 viral protein VP2, which characterizes the subtype 2b. To the best of the authors' knowledge, this report describes the first detection of CPV-2b DNA in tissue fragments from a crab-eating fox.

Indexed as

BrachyuraFeeding BehaviorAge FactorsAnimalsAnimals, WildBrazilCanidaeFemaleParvoviridae InfectionsParvovirus, CanineRetrospective StudiesCerdocyon thousCPV-2bHeartWild

Identifiers

PMID33095410
PMCPMC7966652

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.