ArticleScientific reports2020
Genome-wide identification of AP2/ERF transcription factor-encoding genes in California poppy (Eschscholzia californica) and their expression profiles in response to methyl jasmonate.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 33 citations in OpenAlex.
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- Genome- and Transcriptome-Wide Characterization of AP2/ERF Transcription Factor Superfamily Reveals Their Relevance inPlants (Basel, Switzerland) · 2026Article
- Genome-wide characterization of the AP2/ERF gene family inFrontiers in plant science · 2026Article
- Wounding and Phospholipase C Inhibition: Evaluation of the Alkaloid Profiling in Opium Poppy.Plants (Basel, Switzerland) · 2025Article
- Genome-Wide Identification and Functional Analysis ofPlants (Basel, Switzerland) · 2025Article
- Genome-wide identification, structural characterization and expression profiling of AP2/ERF gene family in bayberry (Myrica rubra).BMC plant biology · 2024Article
- Genome-wide identification of AP2/ERF gene family in Coptis Chinensis Franch reveals its role in tissue-specific accumulation of benzylisoquinoline alkaloids.BMC genomics · 2024Article
- Comparative transcriptional analysis of Persea americana MYB, WRKY and AP2/ERF transcription factors following Phytophthora cinnamomi infection.Molecular plant pathology · 2024Article
- Genome-wide analysis of theFrontiers in plant science · 2024Article
- Unraveling the genetic basis of oil quality in olives: a comparative transcriptome analysis.Frontiers in plant science · 2024Article
- California poppy (Frontiers in plant science · 2023Review
- Genome-Wide Identification and Expression Pattern of theBiology · 2022Article
- Interplay of transcription factors orchestrating the biosynthesis of plant alkaloids.3 Biotech · 2022Review
- Using FIBexDB for In-Depth Analysis of Flax Lectin Gene Expression in Response toPlants (Basel, Switzerland) · 2022Article
- Natural products of medicinal plants: biosynthesis and bioengineering in post-genomic era.Horticulture research · 2022Article
- Jasmonate-Responsive Transcription Factors NnWRKY70a and NnWRKY70b Positively Regulate Benzylisoquinoline Alkaloid Biosynthesis in Lotus (Frontiers in plant science · 2022Article
- Genome-Wide Analysis and Expression Profiles of Ethylene Signal Genes and Apetala2/Ethylene-Responsive Factors in Peanut (Frontiers in plant science · 2022Article
- Transcription Factors in Alkaloid Engineering.Biomolecules · 2021Review
- Genome-Wide Identification and Characterization ofInternational journal of molecular sciences · 2021Article
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4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
With respect to the biosynthesis of plant alkaloids, that of benzylisoquinoline alkaloids (BIAs) has been the most investigated at the molecular level. Previous investigations have shown that the biosynthesis of BIAs is comprehensively regulated by WRKY and bHLH transcription factors, while promoter analyses of biosynthesis enzyme-encoding genes have also implicated the involvement of members of the APETALA2/ethylene responsive factor (AP2/ERF) superfamily. To investigate the physiological roles of AP2/ERF transcription factors in BIA biosynthesis, 134 AP2/ERF genes were annotated using the draft genome sequence data of Eschscholzia californica (California poppy) together with transcriptomic data. Phylogenetic analysis revealed that these genes could be classified into 20 AP2, 5 RAV, 47 DREB, 60 ERF and 2 Soloist family members. Gene structure, conserved motif and orthologous analyses were also carried out. Gene expression profiling via RNA sequencing in response to methyl jasmonate (MeJA) indicated that approximately 20 EcAP2/ERF genes, including 10 group IX genes, were upregulated by MeJA, with an increase in the expression of the transcription factor-encoding gene EcbHLH1 and the biosynthesis enzyme-encoding genes Ec6OMT and EcCYP719A5. Further quantitative RT-PCR confirmed the MeJA responsiveness of the EcAP2/ERF genes, i.e., the increased expression of 9 group IX, 2 group X and 2 group III ERF subfamily genes. Transactivation activity of group IX EcAP2/ERFs was also confirmed by a luciferase reporter assay in conjunction with the promoters of the Ec6OMT and EcCYP719A5 genes. The physiological roles of AP2/ERF genes in BIA biosynthesis and their evolution in the regulation of alkaloid biosynthesis are discussed.
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