ArticleScientific reports2020
Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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21 citing papers in PubMed, 77 citations in OpenAlex.
- Engineering monosex and sterile fish for food production: from conventional methods to CRISPR-based precision.Advanced biotechnology · 2026Review
- The DNA mismatch repair protein Msh4 is essential for meiosis of male but not for female in zebrafish.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026Article
- Governance Perspectives on Genetically Modified Animals for Agriculture and Aquaculture: Challenges for the Assessment of Environmental Risks and Broader Societal Concerns.Animals : an open access journal from MDPI · 2025Review
- Advancing genetic improvement in the omics era: status and priorities for United States aquaculture.BMC genomics · 2025Review
- Emerging challenges in aquaculture: Current perspectives and human health implications.Veterinary world · 2025Review
- Chromosomal aberrations and early mortality in a non-mammalian vertebrate: example from pressure-induced triploid Atlantic salmon.Heredity · 2024Article
- CRISPR/Cas9 Technology for Enhancing Desirable Traits of Fish Species in Aquaculture.International journal of molecular sciences · 2024Review
- Review
- Loss of Fshr Prevents Testicular Maturation in Atlantic Salmon (Salmo salar L.).Endocrinology · 2024Article
- Review
- A homology guide for Pacific salmon genusEcology and evolution · 2023Article
- Identification of fish spermatogenic cells through high-throughput immunofluorescence against testis with an antibody set.Frontiers in endocrinology · 2023Article
- Infertility control of transgenic fluorescent zebrafish with targeted mutagenesis of theFrontiers in genetics · 2023Article
- Impact of germ cell ablation on the activation of the brain-pituitary-gonadal axis in precocious Atlantic salmon (Salmo salar L.) males.Molecular reproduction and development · 2022Article
- Spinyhead Croaker Germ Cells GeneLife (Basel, Switzerland) · 2022Article
- Biotechnology can help us save the genetic heritage of salmon and other aquatic species.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Production of Germ Cell-Less Rainbow Trout by dead end Gene Knockout and their Use as Recipients for Germ Cell Transplantation.Marine biotechnology (New York, N.Y.) · 2022Article
- Sustainable use of CRISPR/Cas in fish aquaculture: the biosafety perspective.Transgenic research · 2022Review
- The Piwil1 N domain is required for germ cell survival in Atlantic salmon.Frontiers in cell and developmental biology · 2022Article
- Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors.BMC genomics · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic introgression of escaped farmed Atlantic salmon (Salmo salar) into wild populations is a major environmental concern for the salmon aquaculture industry. Using sterile fish in commercial aquaculture operations is, therefore, a sustainable strategy for bio-containment. So far, the only commercially used methodology for producing sterile fish is triploidization. However, triploid fish are less robust. A novel approach in which to achieve sterility is to produce germ cell-free salmon, which can be accomplished by knocking out the dead-end (dnd) gene using CRISPR-Cas9. The lack of germ cells in the resulting dnd crispants, thus, prevents reproduction and inhibits subsequent large-scale production of sterile fish. Here, we report a rescue approach for producing germ cells in Atlantic salmon dnd crispants. To achieve this, we co-injected the wild-type (wt) variant of salmon dnd mRNA together with CRISPR-Cas9 constructs targeting dnd into 1-cell stage embryos. We found that rescued one-year-old fish contained germ cells, type A spermatogonia in males and previtellogenic primary oocytes in females. The method presented here opens a possibility for large-scale production of germ-cell free Atlantic salmon offspring through the genetically sterile broodstock which can pass the sterility trait on the next generation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.