Evidence map›Paper›PMID 33093473›Full record

ArticleScientific reports2020

Mode of induction of platelet-derived extracellular vesicles is a critical determinant of their phenotype and function.

P M Ferreira, E Bozbas, S D Tannetta, N Alroqaiba, R Zhou, J T B Crawley, J M Gibbins, C I Jones, J Ahnström, P Yaqoob

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

  1. Trial
  2. Article
  3. Article
  4. Article
  5. Review
  6. Review
  7. Article
  8. Review
  9. Review
  10. Platelets in COVID-19 disease: friend, foe, or both?Pharmacological reports : PR · 2022
    Review
  11. Review
  12. Review
  13. Review
  14. Article
  15. Review
  16. Review
  17. Progress of Platelet Derivatives for Cartilage Tissue Engineering.Frontiers in bioengineering and biotechnology · 2022
    Review
  18. Review
  19. Short-Term High-Intensity Treadmill Exercise Promotes Ceramide-Dependent Extracellular Vesicle Secretion in the Central Nervous System of Mice.Medical science monitor : international medical journal of experimental and clinical research · 2021
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

P M FerreiraSchool of Chemistry, Food and Pharmacy, University of Reading, Reading, RG6 6AP, UK.
E BozbasSchool of Chemistry, Food and Pharmacy, University of Reading, Reading, RG6 6AP, UK.
S D TannettaSchool of Chemistry, Food and Pharmacy, University of Reading, Reading, RG6 6AP, UK.
N AlroqaibaSchool of Chemistry, Food and Pharmacy, University of Reading, Reading, RG6 6AP, UK.
R ZhouSchool of Chemistry, Food and Pharmacy, University of Reading, Reading, RG6 6AP, UK.
J T B CrawleyDepartment of Immunology and Inflammation, Centre for Haematology, Imperial College London, London, UK.
J M GibbinsInstitute for Cardiovascular & Metabolic Research, School of Biological Sciences, University of Reading, Reading, UK.
C I JonesInstitute for Cardiovascular & Metabolic Research, School of Biological Sciences, University of Reading, Reading, UK.
J AhnströmDepartment of Immunology and Inflammation, Centre for Haematology, Imperial College London, London, UK.
P YaqoobSchool of Chemistry, Food and Pharmacy, University of Reading, Reading, RG6 6AP, UK. p.yaqoob@reading.ac.uk.

Funding

Biotechnology and Biological Sciences Research Council BB/N021185/1British Heart Foundation PG/16/36/31967British Heart Foundation RG/15/2/31224British Heart Foundation RG/18/3/33405
6 · The paper itself

Abstract

Platelet-derived extracellular vesicles (PDEVs) are the most abundant amongst all types of EVs in the circulation. However, the mechanisms leading to PDEVs release, their role in coagulation and phenotypic composition are poorly understood. PDEVs from washed platelets were generated using different stimuli and were characterised using nanoparticle tracking analysis. Procoagulant properties were evaluated by fluorescence flow cytometry and calibrated automated thrombography. EVs from plasma were isolated and concentrated using a novel protocol involving a combination of size exclusion chromatography and differential centrifugation, which produces pure and concentrated EVs. Agonist stimulation enhanced PDEV release, but did not alter the average size of EVs compared to those produced by unstimulated platelets. Agonist stimulation led to lower negatively-charged phospholipid externalization in PDEVs, which was reflected in the lower procoagulant activity compared to those generated without agonist stimulation. Circulating EVs did not have externalized negatively-charged phospholipids. None of the 4 types of EVs presented tissue factor. The mechanism by which PDEV formation is induced is a critical determinant of its phenotype and function. Importantly, we have developed methods to obtain clean, concentrated and functional EVs derived from platelet-free plasma and washed platelets, which can be used to provide novel insight into their biological functions.

Indexed as

Blood CoagulationBlood PlateletsAdolescentAdultChromatography, GelExtracellular VesiclesFemaleFlow CytometryFluorescenceHumansMaleMiddle AgedNanoparticlesPhenotypePhospholipidsYoung AdultPhospholipids

Identifiers

PMID33093473
PMCPMC7582134

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.