ArticleClinical epigenetics2020
Smoking-related changes in DNA methylation and gene expression are associated with cardio-metabolic traits.
Article in Clinical epigenetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 53 citations in OpenAlex.
- ATAD3B and SKIL polymorphisms associated with antipsychotic-induced QTc interval change in patients with schizophrenia: a genome-wide association study.Translational psychiatry · 2022Trial
- Environmental Exposures and Epigenetic Remodeling in Supraventricular Tachycardias: What Atrial Fibrillation Can and Cannot Tell Us.Life (Basel, Switzerland) · 2026Review
- Identification of DNA methylation signatures of smoking in women and associations with cardiovascular disease mortality.Communications biology · 2026Article
- Association of DNA methylation with hypertension and blood pressure: a 7-year longitudinal study from KORA F4/FF4.BMC medicine · 2026Article
- Powerful large scale inference in high dimensional mediation analysis.PLoS computational biology · 2026Article
- Speeding up interval estimation for R2-based mediation effect of high-dimensional mediators via cross-fitting.Biostatistics (Oxford, England) · 2025Article
- Immune Signatures of Smoking: Cytokine and Immunoglobulin Dysregulation and Partial Reversibility in a Population-Based Study.Journal of clinical immunology · 2025Article
- Epigenetic aging acceleration among World Trade Center-exposed community members.Scientific reports · 2025Article
- Improving Cardiovascular Health Through the Consideration of Social Factors in Genetics and Genomics Research: A Scientific Statement From the American Heart Association.Circulation. Cardiovascular quality and outcomes · 2025Review
- Environmental pollutants and atherosclerosis: Epigenetic mechanisms linking genetic risk and disease.Atherosclerosis · 2025Review
- Three generations of epigenetic clocks in mediating the adverse effect of smoking on metabolic health.Epigenomics · 2025Article
- Epigenetics of Chronic Stress: Mechanisms and Health Impacts.Current genomics · 2025Review
- Smoking-Associated Changes in Gene Expression in Coronary Artery Disease Patients Using Matched Samples.Current issues in molecular biology · 2024Article
- DNA methylation at AHRR as a master predictor of smoke exposure and a biomarker for sleep and exercise.Clinical epigenetics · 2024Article
- Albendazole ameliorates aerobic glycolysis in myofibroblasts to reverse pulmonary fibrosis.Journal of translational medicine · 2024Article
- Speeding up interval estimation forbioRxiv : the preprint server for biology · 2024Article
- Gene-environment interactions within a precision environmental health framework.Cell genomics · 2024Review
- Cigarette smoking and cardiovascular disease incidence and all-cause mortality: the modifying role of diet quality.BMC public health · 2024Article
- A prospective study of smoking-related white blood cell DNA methylation markers and risk of bladder cancer.European journal of epidemiology · 2024Article
- The onset and the development of cardiometabolic aging: an insight into the underlying mechanisms.Frontiers in pharmacology · 2024Review
Corrections and comments
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTobacco smoking is a well-known modifiable risk factor for many chronic diseases, including cardiovascular disease (CVD). One of the proposed underlying mechanism linking smoking to disease is via epigenetic modifications, which could affect the expression of disease-associated genes. Here, we conducted a three-way association study to identify the relationship between smoking-related changes in DNA methylation and gene expression and their associations with cardio-metabolic traits.
resultsWe selected 2549 CpG sites and 443 gene expression probes associated with current versus never smokers, from the largest epigenome-wide association study and transcriptome-wide association study to date. We examined three-way associations, including CpG versus gene expression, cardio-metabolic trait versus CpG, and cardio-metabolic trait versus gene expression, in the Rotterdam study. Subsequently, we replicated our findings in The Cooperative Health Research in the Region of Augsburg (KORA) study. After correction for multiple testing, we identified both cis- and trans-expression quantitative trait methylation (eQTM) associations in blood. Specifically, we found 1224 smoking-related CpGs associated with at least one of the 443 gene expression probes, and 200 smoking-related gene expression probes to be associated with at least one of the 2549 CpGs. Out of these, 109 CpGs and 27 genes were associated with at least one cardio-metabolic trait in the Rotterdam Study. We were able to replicate the associations with cardio-metabolic traits of 26 CpGs and 19 genes in the KORA study. Furthermore, we identified a three-way association of triglycerides with two CpGs and two genes (GZMA; CLDND1), and BMI with six CpGs and two genes (PID1; LRRN3). Finally, our results revealed the mediation effect of cg03636183 (F2RL3), cg06096336 (PSMD1), cg13708645 (KDM2B), and cg17287155 (AHRR) within the association between smoking and LRRN3 expression.
conclusionsOur study indicates that smoking-related changes in DNA methylation and gene expression are associated with cardio-metabolic risk factors. These findings may provide additional insights into the molecular mechanisms linking smoking to the development of CVD.
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