Evidence map›Paper›PMID 33092644›Full record

ArticleBiological research2020

LncRNA OIP5-AS1 upregulates snail expression by sponging miR-34a to promote ovarian carcinoma cell invasion and migration.

Xingzhi Jiang, Zhongxue Ye, Yafen Jiang, Wen Yu, Qian Fang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biological research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 1 pooled it
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 1 synthesis or guideline pooled it, 21 citations in OpenAlex.

  1. Pooled it
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  6. The role of lncRNA OIP5-AS1 in cancer development and progression.Apoptosis : an international journal on programmed cell death · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Xingzhi JiangDepartment of Gynaecology, Hwa Mei Hospital, University of Chinese Academy of Sciences, No. 41 Northeast Street, Haishu District, Ningbo, 315000, Zhejiang, People's Republic of China. xingzhijiang1997@163.com.ORCID http://orcid.org/0000-0001-5556-6261
Zhongxue YeDepartment of Gynaecology, Hwa Mei Hospital, University of Chinese Academy of Sciences, No. 41 Northeast Street, Haishu District, Ningbo, 315000, Zhejiang, People's Republic of China.
Yafen JiangDepartment of Gynaecology, Hwa Mei Hospital, University of Chinese Academy of Sciences, No. 41 Northeast Street, Haishu District, Ningbo, 315000, Zhejiang, People's Republic of China.
Wen YuDepartment of Gynaecology, Hwa Mei Hospital, University of Chinese Academy of Sciences, No. 41 Northeast Street, Haishu District, Ningbo, 315000, Zhejiang, People's Republic of China.
Qian FangDepartment of Gynaecology, Hwa Mei Hospital, University of Chinese Academy of Sciences, No. 41 Northeast Street, Haishu District, Ningbo, 315000, Zhejiang, People's Republic of China.
University of Chinese Academy of Sciences · CNNingbo No. 2 Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAlthough OIP5-AS1 has been characterized as an oncogenic lncRNA in many types of cancer, its role and underlying mechanism in ovarian carcinoma (OC) remains unknown. This study aimed to investigate the role of OIP5-AS1 in OC.

methodsOC tissues and non-tumor tissues (ovary tissues within 3 cm around tumors) were collected from 58 OC patients (age range 36 to 67 years old, mean age 51.4 ± 5.9 years old). The expression of OIP5-AS1 and snail in paired tissues were determined by RT-qPCR. The interaction between OIP5-AS1 and miR-34a was predicted by IntaRNA2.0 and confirmed by dual luciferase reporter assay. The effects of overexpression of OIP5-AS1 and miR-34a on the expression of snail were analyzed by RT-qPCR and Western blotting. Cell invasion and migration were analyzed by Transwell assay.

resultsWe observed that the expression of OIP5-AS1 and snail was upregulated and positively correlated with each other in OC. RNA-RNA interaction analysis showed that OIP5-AS1 might sponge miR-34a. In OC cells, overexpression of OIP5-AS1 resulted in the upregulated expression of snail, while overexpression of miR-34a downregulated the expression of snail. In addition, overexpression of miR-34a reduced the effects of overexpression of OIP5-AS1 on the expression of snail. In cell invasion and migration assay, overexpression of OIP5-AS1 and snail resulted in increased OC cell invasion and migration, while overexpression of miR-34a decreased OC cell invasion and migration. Moreover, overexpression of miR-34a attenuated the effects of OIP5-AS1 overexpression on OC cell invasion and migration.

conclusionsTherefore, OIP5-AS1 may upregulate snail expression in OC by sponging miR-34a to promote OC cell invasion and migration.

Indexed as

MicroRNAsOvarian NeoplasmsRNA, Long NoncodingAdultAgedCell ProliferationFemaleGene Expression Regulation, NeoplasticHumansMiddle AgedNeoplasm Invasivenesslong noncoding RNA OIP5, humanMicroRNAsMIRN34 microRNA, humanRNA, Long NoncodingmiR-34aOCOIP5-AS1Snail

Identifiers

PMID33092644
PMCPMC7579860
OpenAlexW3094529961

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.