ArticlePloS one2020
Screening for modulators of neural network activity in 3D human iPSC-derived cortical spheroids.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- Detecting MUNC18-1 related presynaptic dysfunction and rescue in human iPSC-derived neurons.Scientific reports · 2025Article
- Geometrical constraints dictate assembly and phenotype of human iPSC-derived motoneuronal spheroids.Stem cell research & therapy · 2025Article
- Article
- Imaging 3D cell cultures with optical microscopy.Nature methods · 2025Review
- A 3D human iPSC-derived multi-cell type neurosphere system to model cellular responses to chronic amyloidosis.Journal of neuroinflammation · 2025Article
- Investigating Connectivity Deficits in Alzheimer's Disease Using a Novel 3D Bioprinted Model Designed to Quantify Neurite Outgrowth.Bioengineering (Basel, Switzerland) · 2025Article
- Stimulus effects of extremely low-frequency electric field exposure on calcium oscillations in a human cortical spheroid.Bioelectromagnetics · 2025Article
- In vitro electrophysiological drug testing on neuronal networks derived from human induced pluripotent stem cells.Stem cell research & therapy · 2024Article
- Modulation of CaScientific reports · 2024Article
- Human stem cell-based models to study synaptic dysfunction and cognition in schizophrenia: A narrative review.Schizophrenia research · 2024Review
- Harnessing the potential of human induced pluripotent stem cells, functional assays and machine learning for neurodevelopmental disorders.Frontiers in neuroscience · 2024Review
- Stem cell-based approaches for developmental neurotoxicity testing.Frontiers in toxicology · 2024Review
- Functional brain region-specific neural spheroids for modeling neurological diseases and therapeutics screening.Communications biology · 2023Article
- Orange/far-red hybrid voltage indicators with reduced phototoxicity enable reliable long-term imaging in neurons and cardiomyocytes.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- A multiparametric calcium signal screening platform using iPSC-derived cortical neural spheroids.SLAS discovery : advancing life sciences R & D · 2022Article
- Review
- Non-permissive SARS-CoV-2 infection in human neurospheres.Stem cell research · 2021Article
- Non-permissive SARS-CoV-2 infection in human neurospheres.bioRxiv : the preprint server for biology · 2021Article
- Human iPSC-Derived 2D and 3D Platforms for Rapidly Assessing Developmental, Functional, and Terminal Toxicities in Neural Cells.International journal of molecular sciences · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human induced Pluripotent Stem Cells (iPSCs) are a powerful tool to dissect the biology of complex human cell types such as those of the central nervous system (CNS). However, robust, high-throughput platforms for reliably measuring activity in human iPSC-derived neuronal cultures are lacking. Here, we assessed 3D cultures of cortical neurons and astrocytes displaying spontaneous, rhythmic, and highly synchronized neural activity that can be visualized as calcium oscillations on standard high-throughput fluorescent readers as a platform for CNS-based discovery efforts. Spontaneous activity and spheroid structure were highly consistent from well-to-well, reference compounds such as TTX, 4-AP, AP5, and NBQX, had expected effects on neural spontaneous activity, demonstrating the presence of functionally integrated neuronal circuitry. Neurospheroid biology was challenged by screening the LOPAC®1280 library, a collection of 1280 pharmacologically active small molecules. The primary screen identified 111 compounds (8.7%) that modulated neural network activity across a wide range of neural and cellular processes and 16 of 17 compounds chosen for follow-up confirmed the primary screen results. Together, these data demonstrate the suitability and utility of human iPSC-derived neurospheroids as a screening platform for CNS-based drug discovery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.