ArticleAnalytical chemistry2020
Fingerprinting Bacterial Metabolic Response to Erythromycin by Raman-Integrated Mid-Infrared Photothermal Microscopy.
Article in Analytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Mid-Infrared Photothermal Mesoscopy with Millimeter Field of View and Sub-micron Spatial Resolution.bioRxiv : the preprint server for biology · 2025Article
- Background-Free Mid-Infrared Photothermal Microscopy via Single-Shot Measurement of Thermal Decay.Analytical chemistry · 2025Article
- Heat application in live cell imaging.FEBS open bio · 2024Review
- A tutorial on optical photothermal infrared (O-PTIR) microscopy.APL photonics · 2024Article
- Overtone photothermal microscopy for high-resolution and high-sensitivity vibrational imaging.Nature communications · 2024Article
- Mid-infrared Photothermal Imaging: Instrument and Life Science Applications.Analytical chemistry · 2024Review
- Roadmap on Label-Free Super-Resolution Imaging.Laser & photonics reviews · 2023Article
- Far-field super-resolution chemical microscopy.Light, science & applications · 2023Review
- Mid-Infrared Photothermal-Fluorescence In Situ Hybridization for Functional Analysis and Genetic Identification of Single Cells.Analytical chemistry · 2023Article
- Optical photothermal infrared spectroscopy: A novel solution for rapid identification of antimicrobial resistance at the single-cell levelFrontiers in microbiology · 2023Article
- High-sensitivity hyperspectral vibrational imaging of heart tissues by mid-infrared photothermal microscopy.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2022Article
- Simultaneous Raman and infrared spectroscopy: a novel combination for studying bacterial infections at the single cell level.Chemical science · 2022Article
- Simultaneous Raman and Infrared Spectroscopy of Stable Isotope LabelledSensors (Basel, Switzerland) · 2022Article
- Nanosecond-resolution photothermal dynamic imaging via MHZ digitization and match filtering.Nature communications · 2021Article
- Fluorescence-Detected Mid-Infrared Photothermal Microscopy.Journal of the American Chemical Society · 2021Article
- Bond-selective imaging by optically sensing the mid-infrared photothermal effect.Science advances · 2021Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
Abstract
We report rapid and sensitive phenotyping of bacterial response to antibiotic treatment at single-cell resolution by a Raman-integrated optical mid-infrared photothermal (MIP) microscope. The MIP microscope successfully detected biochemical changes of bacteria in specific to the acting mechanism of erythromycin with 1 h incubation. Compared to Raman spectroscopy, MIP spectroscopy showed a much larger signal-to-noise ratio at the fingerprint region at an acquisition speed as fast as 1 s per spectrum. The high sensitivity of MIP enabled detection of metabolic changes at antibiotic concentrations below minimum inhibitory concentration (MIC). Meanwhile, the single-cell resolution of the technique allowed observation of heteroresistance within one bacterial population, which is of great clinical relevance. This study showcases characterizing antibiotic response as one of the many possibilities of applying MIP microscopy to single-cell biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.