ArticlePlant biotechnology journal2021
Three AP2/ERF family members modulate flavonoid synthesis by regulating type IV chalcone isomerase in citrus.
Article in Plant biotechnology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
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91 citing papers in PubMed, 194 citations in OpenAlex.
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- An AcMYB10-AcF3GT1/GST1 module restricts anthocyanin synthesis in the fruit flesh of kiwifruit.Plant cell reports · 2026Article
- Dynamic Coordination: How ERF Transcription Factors Coordinate Plant Development and Adaptive Stress Responses.Biomolecules · 2026Review
- Molecular insights into the regulation of flavonoid biosynthesis in fruits.Horticulture research · 2026Article
- The Tomato Transcription Factor RAV Affects the Systemic Infection of TYLCV by Interacting With V2.Molecular plant pathology · 2026Article
- Identification and functional validation of hub genes responding to waterlogging stress during the filling period in quinoa (Chenopodium Quinoa Willd.).BMC plant biology · 2026Article
- Identification and analysis of the AP2/ERF gene family inFrontiers in plant science · 2026Article
- AP2/ERF transcription factors regulate the biosynthesis of terpenoids, phenolics, and alkaloids in plants.Horticulture research · 2026Article
- Genome-wide characterization of the AP2/ERF gene family inFrontiers in plant science · 2026Article
- Integrated multi-omics elucidates StAN1 as a key regulator reprogramming flavonoid partitioning and co-regulatory networks in potato tubers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Metabolomics and Transcriptomic Analysis Revealed the Response Mechanism of Maize to Saline-Alkali Stress.Plant biotechnology journal · 2025Article
- Multi-omics analyses reveal the effects of layerage and grafting on flavonoid synthesis and accumulation inHorticulture research · 2025Article
- Integrated Transcriptome-Metabolome Analysis Reveals the Flavonoids Metabolism Mechanism of Maize Radicle in Response to Low Temperature.Plants (Basel, Switzerland) · 2025Article
- Glycyrrhiza, a commonly used medicinal herb: Review of species classification, pharmacology, active ingredient biosynthesis, and synthetic biology.Journal of advanced research · 2025Review
- ERF100 regulated by ERF28 and NOR controls pectate lyase 7, modulating fig (Ficus carica L.) fruit softening.Plant biotechnology journal · 2025Article
- Genome-Wide Analysis of the FNSII Gene Family and the Role of CitFNSII-1 in Flavonoid Synthesis in Citrus.Plants (Basel, Switzerland) · 2025Article
- VqERF1B-VqERF062-VqNSTS2 transcriptional cascade enhances stilbene biosynthesis and resistance to powdery mildew in grapevine.Plant biotechnology journal · 2025Article
31 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flavanones and flavones are excellent source of bioactive compounds but the molecular basis of their highly efficient production remains elusive. Chalcone isomerase (CHI) family proteins play essential roles in flavonoid biosynthesis but little are known about the transcription factors controlling their gene expression. Here, we identified a type IV CHI (designated as CitCHIL1) from citrus which enhances the accumulation of citrus flavanones and flavones (CFLs). CitCHIL1 participates in a CFL biosynthetic metabolon and assists the cyclization of naringenin chalcone to (2S)-naringenin, which leads to the efficient influx of substrates to chalcone synthase (CHS) and improves the catalytic efficiency of CHS. Overexpressing CitCHIL1 in Citrus and Arabidopsis significantly increased flavonoid content and RNA interference-induced silencing of CitCHIL1 in citrus led to a 43% reduction in CFL content. Three AP2/ERF transcription factors were identified as positive regulators of the CitCHIL1 expression. Of these, two dehydration-responsive element binding (DREB) proteins, CitERF32 and CitERF33, activated the transcription by directly binding to the CGCCGC motif in the promoter, while CitRAV1 (RAV: related to ABI3/VP1) formed a transcription complex with CitERF33 that strongly enhanced the activation efficiency and flavonoid accumulation. These results not only illustrate the specific function that CitCHIL1 executes in CFL biosynthesis but also reveal a new DREB-RAV transcriptional complex regulating flavonoid production.
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