Evidence map›Paper›PMID 33089439›Full record

ArticleJournal of assisted reproduction and genetics2020

The effect of laser-assisted hatching on the methylation and expression pattern of imprinted gene IGF2/H19 in mouse blastocysts and offspring.

Peng Huo, Kai Deng, Lulu Wang, Man Li, Jun Yao, Jianghua Le, Xiaocan Lei, Shun Zhang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 87% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Peng Huo *School of Public and Health, Guilin Medical University, Guilin, 541004, China.
Kai Deng *Guangxi High Education Key Laboratory for Animal Reproduction and Biotechnology, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, China.
Lulu WangGuangxi High Education Key Laboratory for Animal Reproduction and Biotechnology, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, China.
Man LiDepartment of Reproductive Medical Center, The Affiliated Hospital of Guilin Medical University, Guilin, 541001, China.
Jun YaoDepartment of Reproductive Medical Center, The Affiliated Hospital of Guilin Medical University, Guilin, 541001, China.
Jianghua LeDepartment of Reproductive Medical Center, The Affiliated Hospital of Guilin Medical University, Guilin, 541001, China.
Xiaocan LeiClinical Anatomy & Reproductive Medicine Application Institute, Department of Histology and Embryology, University of South China, Hengyang, 421001, China. 2019000013@usc.edu.cn.
Shun ZhangDepartment of Reproductive Medical Center, The Affiliated Hospital of Guilin Medical University, Guilin, 541001, China. artzhangshun@glmc.edu.cn.
Guilin Medical University · CNGuangxi University · CNUniversity of South China · CN

Funding

China National Natural Science Fund 81960274, 81860285, 81860733Guangxi Science Foundation 2017GXNSFBA198189Key Project of Guangxi Natural Science Foundation 2018GXNSFDA050003
6 · The paper itself

Abstract

purposeThis study aimed to determine the effects of drilling and thinning treatment of laser-assisted hatching on the expression and methylation of imprinted gene IGF2/H19 in embryos and offspring.

methodsThe prehatching blastocysts with treatment of drilling or thinning, or control prehatching blastocysts, were transplanted in surrogate uteri. The DNA methylation of IGF2/H19 imprinting control region (ICR) and the expression of IGF2 and H19 were respectively evaluated using bisulfite conversion-mediated sequencing and real-time PCR.

resultsThe drilling group showed a significant increase in the development rate of hatched blastocysts in comparison with the control and thinning group. DNA methylation level of IGF2/H19 ICR of hatched blastocysts in the thinning group was 27.33% in comparison with the 38.67% and 36% observed in the control and drilling group. The thinning treatment increased the DNA methylation level of IGF2/H19 ICR in the placenta in comparison with the control and drilling group. The drilling and thinning treatment decreased the expression level of H19 mRNA in prehatching and hatched blastocysts as well as placenta, while a significant increase in the expression level of H19 mRNA of offspring was observed in the thinning group. The thinning treatment increased the expression level of IGF2 mRNA of prehatching blastocysts and offspring and a significant decrease in placenta, while the drilling treatment resulted in a significant increase in the expression level of IGF2 mRNA of hatched blastocysts and placenta.

conclusionThese observations suggested that drilling used for hatching of in vitro cultured mouse blastocysts may improve the production of offspring.

Indexed as

DNA MethylationGenomic ImprintingAnimalsAnimals, NewbornBlastocystEmbryo Culture TechniquesFemaleFertilization in VitroInsulin-Like Growth Factor IILasersMaleMicePlacentaPregnancyRNA, Long NoncodingH19 long non-coding RNAInsulin-Like Growth Factor IIRNA, Long NoncodingDrillingExpressionIGF2/H19Laser-assisted hatchingMethylationOffspring

Identifiers

PMID33089439
PMCPMC7714794
OpenAlexW3094200775

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.